Bøgh H O, Willingham A L, Johansen M V, Eriksen L, Christensen N O
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Acta Vet Scand. 1997;38(2):147-55. doi: 10.1186/BF03548494.
An optimized procedure for perfusion of pigs infected with Schistosoma japonicum was developed. The technique involves insertion of a perfusion influx tube into the thoracic descending aorta, clamping vessels to parts of the body which did not need to be perfused (the kidneys, hind legs, etc.) and placing a collection tube directly into the portal vein. In addition, the clamping technique allows for separate perfusion of the liver and intestinal veins. The perfusion medium was a sodium citrate buffer (40 degrees C) to which the vasodilator sodium nitroprusside was added. Furthermore, an experiment was conducted to investigate if the perfusion efficiency, measured by total worm recovery, could be increased if praziquantel was administered prior to perfusion. Twelve pigs were each infected with 1000 S. japonicum cercariae and their schistosomes were collected 11 weeks later by separate perfusion of the liver and intestinal veins. Six of these pigs were treated orally with praziquantel one hour before perfusion. In general, the vessels of the livers and intestines of all pigs were well perfused, judging by the resulting pale colour of the tissues. Worms from praziquantel treated pigs were collected within 5 min of perfusion as opposed to approximately 20 min in the non-treated pigs. More worms were collected from the livers of the praziquantel treated pigs, indicating a hepatic shift of schistosomes from the intestinal mesenteries. However, comparable numbers of worms were retained in the mesenteric veins following perfusion in the 2 groups, indicating that manual recovery of schistosomes from the intestinal mesenteries is necessary in addition to perfusion for obtaining the total worm counts. Another experiment was conducted to determine if the intensity and/or duration of infection had an effect on the number of worms collected by the perfusion technique. Seventy-two pigs were allocated into 3 groups of 24 pigs each, which were infected with either 100, 500 or 2000 cercariae per pig. The 3 groups were further divided into 4 subgroups of 6 pigs each which were perfused with our selective technique at 4, 11, 17 or 24 weeks post infection, respectively. All of the pigs received an oral praziquantel treatment prior to perfusion. The results indicated that increasing intensities and/or duration of infection resulted in trapping of schistosomes in intravascular inflammatory reactions which made it more difficult to collect the adult schistosomes by perfusion.
开发了一种用于对感染日本血吸虫的猪进行灌注的优化程序。该技术包括将灌注流入管插入胸降主动脉，夹住身体不需要灌注的部分（肾脏、后腿等）的血管，并将收集管直接放入门静脉。此外，夹紧技术允许分别灌注肝脏和肠静脉。灌注介质是添加了血管扩张剂硝普钠的柠檬酸钠缓冲液（40摄氏度）。此外，进行了一项实验，以研究如果在灌注前给予吡喹酮，以总虫回收率衡量的灌注效率是否可以提高。12头猪每头感染1000条日本血吸虫尾蚴，11周后通过分别灌注肝脏和肠静脉收集它们的血吸虫。其中6头猪在灌注前1小时口服吡喹酮。总体而言，从组织呈现的苍白颜色判断，所有猪的肝脏和肠道血管都得到了良好的灌注。与未治疗的猪大约20分钟相比，吡喹酮治疗的猪的虫体在灌注后5分钟内被收集。从吡喹酮治疗的猪的肝脏中收集到更多的虫体，表明血吸虫从肠系膜向肝脏转移。然而，两组灌注后肠系膜静脉中保留的虫体数量相当，这表明除了灌注外，还需要手动从肠系膜中回收血吸虫以获得总虫数。进行了另一项实验，以确定感染强度和/或持续时间是否对通过灌注技术收集的虫体数量有影响。72头猪被分为3组，每组24头猪，每头猪分别感染100、500或2000条尾蚴。这3组进一步分为4个亚组，每组6头猪，分别在感染后4、11、17或24周用我们的选择性技术进行灌注。所有猪在灌注前都接受了口服吡喹酮治疗。结果表明，感染强度和/或持续时间的增加导致血吸虫被困在血管内炎症反应中，这使得通过灌注收集成虫血吸虫更加困难。
