Takao K, Nagatani T, Kitamura Y, Kawasaki K, Hayakawa H, Yamawaki S
Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Japan.
Eur J Pharmacol. 1995 Dec 29;294(2-3):721-6. doi: 10.1016/0014-2999(95)00620-6.
We studied the effects of chronic forced swim stress on 5-HT2 receptors and beta-adrenoceptors in the rat frontal cortex. The number of 5-HT2 receptors was increased immediately after the last chronic stress, but not after an acute stress. In vivo, the number of wet-dog shakes induced by a 5-HT2 receptor agonist, (+/-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), was increased 24 h after the last chronic stress. However, the concentrations of 5-HT and 5-hydroxyindole acetic acid (5-HIAA), measured by high pressure liquid chromatography (HPLC), were not altered by this stress. Binding sites for [3H]CGP-12177, i.e., beta-adrenoceptor sites, were unchanged after both the acute and the chronic stress. These results suggest that, in the rat, the chronic forced swim stress increases the number of frontal cortical 5-HT2 receptors and the number of wet-dog shakes mediated by these receptors, while the number of frontal cortical beta-adrenoceptors is not increased by this treatment.
我们研究了慢性强迫游泳应激对大鼠额叶皮质中5-羟色胺2（5-HT2）受体和β-肾上腺素能受体的影响。在最后一次慢性应激后，5-HT2受体的数量立即增加，但急性应激后未增加。在体内，5-HT2受体激动剂（±）-1-（2,5-二甲氧基-4-碘苯基）-2-氨基丙烷（DOI）诱导的湿狗样抖动次数在最后一次慢性应激后24小时增加。然而，通过高压液相色谱（HPLC）测定的5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）的浓度并未因这种应激而改变。急性和慢性应激后，[3H]CGP-12177的结合位点，即β-肾上腺素能受体位点，均未发生变化。这些结果表明，在大鼠中，慢性强迫游泳应激增加了额叶皮质5-HT2受体的数量以及由这些受体介导的湿狗样抖动次数，而这种处理并未增加额叶皮质β-肾上腺素能受体的数量。
