Schindler M, Harrington K A, Humphrey P P, Emson P C
Department of Neurobiology, Babraham Institute, Cambridge, UK.
Brain Res Mol Brain Res. 1995 Dec 28;34(2):321-6. doi: 10.1016/0169-328x(95)00191-t.
Genes for five high affinity somatostatin receptors, named sst1-5, have been cloned recently. In this study we describe the tissue distribution and cellular localisation of mRNA encoding sst1, sst3 and sst4 receptors in the human cerebellum, frontal cortex (Brodmann's area 11) and hippocampus. RT-PCR and in situ hybridisation studies indicated a distinct, but partially overlapping pattern of expression of the receptor mRNAs. In situ hybridisation studies using co-expression techniques with probes for sst1, sst3 and sst4 receptor mRNA on paraffin sections revealed the presence of neurones expressing more than one somatostatin receptor mRNA type in both the hippocampus and pyramidal cells of layer V of the frontal cortex.
最近已克隆出五种高亲和力生长抑素受体的基因，命名为sst1 - 5。在本研究中，我们描述了编码sst1、sst3和sst4受体的mRNA在人小脑、额叶皮质（布罗德曼11区）和海马体中的组织分布及细胞定位。逆转录聚合酶链反应（RT-PCR）和原位杂交研究表明，受体mRNA的表达模式独特，但部分重叠。在石蜡切片上，使用针对sst1、sst3和sst4受体mRNA的探针进行共表达技术的原位杂交研究显示，在海马体和额叶皮质第V层的锥体细胞中均存在表达不止一种生长抑素受体mRNA类型的神经元。
