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来自正常鹅细胞释放颗粒的RNA指导的DNA聚合酶。

RNA-directed DNA polymerase from particles released by normal goose cells.

作者信息

Bauer G, Temin H M

出版信息

J Virol. 1979 Mar;29(3):1006-13. doi: 10.1128/JVI.29.3.1006-1013.1979.

Abstract

Cells from a goose embryo were shown to release particle-associated RNA-directed DNA polymerase and RNase H activities that required the presence of Nonidet P-40 for detection. The particles were not infectious and did not have endogenous DNA synthesis. The goose particle DNA polymerase was related to the DNA polymerase of spleen necrosis virus with respect to size and was inhibited by immunoglobulin G to spleen necrosis virus DNA polymerase. However, goose cells producing DNA polymerase-containing particles did not contain reticuloendotheliosis virus-related nucleotide sequences in their DNA.

摘要

来自鹅胚胎的细胞被证明能释放与颗粒相关的RNA指导的DNA聚合酶和核糖核酸酶H活性,这些活性需要存在去氧胆酸钠(Nonidet P - 40)才能检测到。这些颗粒没有传染性,也没有内源性DNA合成。鹅颗粒DNA聚合酶在大小方面与脾坏死病毒的DNA聚合酶相关,并且被针对脾坏死病毒DNA聚合酶的免疫球蛋白G所抑制。然而,产生含DNA聚合酶颗粒的鹅细胞在其DNA中不包含与网状内皮组织增殖症病毒相关的核苷酸序列。

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