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禽肿瘤病毒脱氧核糖核酸聚合酶的血清学分析。I. 用纯化的脱氧核糖核酸聚合酶制备单特异性抗血清并进行特性鉴定。

Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. I. Preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase.

作者信息

Watson K E, Nowinski R C, Yaniv A, Spiegelman S

出版信息

J Virol. 1972 Nov;10(5):951-8. doi: 10.1128/JVI.10.5.951-958.1972.

DOI:10.1128/JVI.10.5.951-958.1972
PMID:4117965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356564/
Abstract

Monospecific antiserum was prepared against purified deoxyribonucleic acid (DNA) polymerase from avian myeloblastosis virus (AMV). Immunodiffusion assay with purified DNA polymerase revealed that the anti-DNA polymerase serum formed one precipitation band, whereas no reaction with any of the seven major structural proteins of AMV was observed. The antiserum also demonstrated enzyme-neutralizing antibody activity that was associated with the immunoglobulin G fraction. There was no difference in the neutralization of DNA polymerase activity directed by ribonucleic acid (RNA), DNA, or RNA-DNA hybrid templates.

摘要

制备了针对禽成髓细胞瘤病毒（AMV）纯化的脱氧核糖核酸（DNA）聚合酶的单特异性抗血清。用纯化的DNA聚合酶进行免疫扩散试验表明，抗DNA聚合酶血清形成了一条沉淀带，而未观察到与AMV的七种主要结构蛋白中的任何一种发生反应。该抗血清还表现出与免疫球蛋白G组分相关的酶中和抗体活性。在由核糖核酸（RNA）、DNA或RNA-DNA杂交模板指导的DNA聚合酶活性的中和方面没有差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a6/356564/0dde0e8a2a7a/jvirol00275-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a6/356564/e06179af58b5/jvirol00275-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a6/356564/0dde0e8a2a7a/jvirol00275-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a6/356564/e06179af58b5/jvirol00275-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a6/356564/0dde0e8a2a7a/jvirol00275-0075-a.jpg

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Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. I. Preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase.禽肿瘤病毒脱氧核糖核酸聚合酶的血清学分析。I. 用纯化的脱氧核糖核酸聚合酶制备单特异性抗血清并进行特性鉴定。
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引用本文的文献

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2
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Common properties of the oncogenic RNA viruses (oncornaviruses).致癌RNA病毒（肿瘤病毒）的共同特性。
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Binding of tryptophanyl-tRNA to the reverse transcriptase of replication-defective avian sarcoma viruses.色氨酰-tRNA与复制缺陷型禽肉瘤病毒逆转录酶的结合。
J Virol. 1978 Nov;28(2):434-43. doi: 10.1128/JVI.28.2.434-443.1978.
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An RNA-dependent DNA polymerase, different from the known viral reverse transcriptases, in the chicken system.在鸡的系统中，一种依赖RNA的DNA聚合酶，与已知的病毒逆转录酶不同。
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3025-9. doi: 10.1073/pnas.73.9.3025.
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Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase).色氨酸转移RNA与禽成髓细胞瘤病毒RNA依赖性DNA聚合酶（逆转录酶）的特异性结合。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2535-9. doi: 10.1073/pnas.72.7.2535.
10
Screen for type-C ribonucleic acid viruses in vaccines using the ribonucleic acid-dependent deoxyribonucleic acid polymerase assay.使用依赖核糖核酸的脱氧核糖核酸聚合酶测定法检测疫苗中的C型核糖核酸病毒。
J Clin Microbiol. 1975 Apr;1(4):353-8. doi: 10.1128/jcm.1.4.353-358.1975.
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Purification of the DNA polymerase of avian myeloblastosis virus.禽成髓细胞瘤病毒DNA聚合酶的纯化
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Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses.核糖核酸肿瘤病毒逆转录酶的免疫学关系
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Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses.肿瘤病毒蛋白质的色谱分离与抗原分析。I. 禽白血病-肉瘤病毒
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DNA synthesis by RNA-containing tumor viruses.含RNA肿瘤病毒的DNA合成
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Deoxyribonucleic acid polymerase associated with Rous sarcoma virus and avian myeloblastosis virus: properties of the enzyme and its product.与劳氏肉瘤病毒和禽成髓细胞瘤病毒相关的脱氧核糖核酸聚合酶：酶及其产物的特性
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Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses.RNA肿瘤病毒的致癌机制。I. 鼠肉瘤病毒中的一种RNA依赖性DNA聚合酶。
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