Wu K Y, Hong S J, Chung Y M
Department of Ophthalmology, Kaohsiung Medical College, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1996 Jul;12(7):388-93.
It was found that long-term therapy with topical antiglaucoma drugs may increase the number of fibroblasts and inflammatory cells in the conjunctiva and Tenon's capsule then decrease the success of glaucoma filtering surgery. Since some dopamine antagonists and agonists are reported to have ocular hypotensive effects, they could potentially be used as clinical antiglaucoma agents in future. In this study, several dopamine antagonists and agonists were evaluated in their effects on the fibroblast proliferation of ocular tissues with cultured human ocular Tenon's fibroblast cells. It was found that dopamine antagonists inhibited [3H]-thymidine uptake to 10.8%, 14.4%, 42.2% and 72.4% of control in domperidone, 28.6%, 67.7%, 96.9% and 99.2% in haloperidol, 9.8%, 18.9%, 103.5% and 104.6% in moperone and 9.8%, 24.7%, 70.8% and 96.9% in loxapine respectively, while the 0.5% concentration of drugs were dissolved in dimethyl sulfoxide (DMSO) followed by dilution in culture medium to final concentrations ranging from 0.05%, 0.005%, 0.0005% to 0.00005%. In the case of dopamine agonist, bromocriptine, the proliferation of cultured human Tenon's fibroblast cells was also inhibited for 25.1% of the control at 0.05% concentration and 92.3% of the control at 0.00005% concentration. These results indicate that these dopamine drugs have a potential long-term application in the treatment of glaucoma without having the stimulating effect on the proliferation of human Tenon's fibroblast cells.
研究发现，长期使用局部抗青光眼药物治疗可能会增加结膜和Tenon囊中成纤维细胞和炎性细胞的数量，进而降低青光眼滤过手术的成功率。由于据报道一些多巴胺拮抗剂和激动剂具有降眼压作用，它们未来有可能被用作临床抗青光眼药物。在本研究中，使用培养的人眼Tenon成纤维细胞，评估了几种多巴胺拮抗剂和激动剂对眼组织成纤维细胞增殖的影响。结果发现，多巴胺拮抗剂抑制[3H]-胸腺嘧啶核苷摄取，多潘立酮分别为对照的10.8%、14.4%、42.2%和72.4%，氟哌啶醇分别为28.6%、67.7%、96.9%和99.2%，莫哌隆分别为9.8%、18.9%、103.5%和104.6%，洛沙平分别为9.8%、24.7%、70.8%和96.9%，同时将0.5%浓度的药物溶解于二甲基亚砜（DMSO）中，然后在培养基中稀释至最终浓度为0.05%、0.005%、0.0005%至0.00005%。对于多巴胺激动剂溴隐亭，在0.05%浓度时，培养的人Tenon成纤维细胞增殖也被抑制至对照的25.1%，在0.00005%浓度时为对照的92.3%。这些结果表明，这些多巴胺药物在治疗青光眼方面具有潜在的长期应用价值，且对人Tenon成纤维细胞的增殖没有刺激作用。
