Wu K Y, Hong S J, Wang H Z
Department of Ophthalmology, Kaohsiung Medical College, Taiwan, Republic of China.
J Ocul Pharmacol Ther. 1997 Feb;13(1):13-21. doi: 10.1089/jop.1997.13.13.
It has been found that long-term therapy with topical antiglaucoma drugs may decrease the success of glaucoma filtering surgery. In this study, various antiglaucoma drugs, including carteolol, betaxolol, levobunolol, pilocarpine, and timolol, were investigated for their proliferative effect on cultured human Tenon's fibroblast cells. It was found that the 3H-thymidine uptake of cultured fibroblast cells was inhibited by commercial antiglaucoma drugs, including carteolol of 0.1% concentration (13%), 0.01% (50%) and 0.001% (53%), betaxolol of 0.05% concentration (14%), 0.005% (42%) and 0.0005% (62%), levobunolol of 0.05% concentration (2%), 0.005% (32%) and 0.0005% (55%), and timolol of 0.025% concentration (4%), 0.0025% (47%) and 0.00025% (55%), whereas the 3H-thymidine uptake was increased by commercial pilocarpine eyedrop from 103% (0.2% of concentration), 170% (0.02% of concentration) to 171% (0.002% of concentration), when cells were treated with commercial drugs for 100 min. Meanwhile, the proliferations of cultured fibroblast cells were stimulated by simultaneously combining 0.2%, 0.02% and 0.002% concentrations of pilocarpine eyedrops with other antiglaucoma drugs, such as carteolol of 0.1% concentration (50%), 0.01% (113%) and 0.001% (138%), betaxolol of 0.05% concentration (24%), 0.005% (128%) and 0.0005% (142%), levobunolol of 0.05% concentration (32%), 0.005% (87%) and 0.0005% (119%), and timolol of 0.025% concentration (15%), 0.0025% (94%) and 0.00025% (118%). Following incubation with pure pilocarpine, the proliferation of Tenon's fibroblast cells was inhibited for 84% (0.2% concentration), 84% (0.02% concentration) and 90% (0.002% concentration). When fibroblast cells were treated with commercial pilocarpine eyedrops for 24 hours, the 3H-thymidine uptake was increased to 160% (0.02% concentration) and 172% (0.002% concentration). This result shows that the commercial pilocarpine may play a crucial role for the proliferation of cultured human Tenon's fibroblast cells.
已发现局部抗青光眼药物的长期治疗可能会降低青光眼滤过手术的成功率。在本研究中，研究了包括卡替洛尔、倍他洛尔、左布诺洛尔、毛果芸香碱和噻吗洛尔在内的各种抗青光眼药物对培养的人Tenon成纤维细胞的增殖作用。结果发现，市售抗青光眼药物可抑制培养的成纤维细胞对³H-胸腺嘧啶核苷的摄取，包括浓度为0.1%的卡替洛尔（抑制13%）、0.01%（抑制50%）和0.001%（抑制53%），浓度为0.05%的倍他洛尔（抑制14%）、0.005%（抑制42%）和0.0005%（抑制62%），浓度为0.05%的左布诺洛尔（抑制2%）、0.005%（抑制32%）和0.0005%（抑制55%），以及浓度为0.025%的噻吗洛尔（抑制4%）、0.0025%（抑制47%）和0.00025%（抑制55%）；而当用市售药物处理细胞100分钟时，市售毛果芸香碱滴眼液可使³H-胸腺嘧啶核苷摄取增加，从浓度为0.2%时增加103%、0.02%时增加170%至0.002%时增加171%。同时，将浓度为0.2%、0.02%和0.002%的毛果芸香碱滴眼液与其他抗青光眼药物同时使用，可刺激培养的成纤维细胞增殖，如浓度为0.1%的卡替洛尔（增加50%）、0.01%（增加113%）和0.001%（增加138%），浓度为0.05%的倍他洛尔（增加24%）、0.005%（增加128%）和0.0005%（增加142%），浓度为0.05%的左布诺洛尔（增加32%）、0.005%（增加87%）和0.0005%（增加119%），以及浓度为0.025%的噻吗洛尔（增加15%）、0.0025%（增加94%）和0.00025%（增加118%）。用纯毛果芸香碱孵育后，Tenon成纤维细胞的增殖受到抑制，浓度为0.2%时抑制84%、0.02%时抑制84%、0.002%时抑制90%。当成纤维细胞用市售毛果芸香碱滴眼液处理24小时时，³H-胸腺嘧啶核苷摄取增加到浓度为0.02%时增加160%、0.002%时增加172%。这一结果表明，市售毛果芸香碱可能对培养的人Tenon成纤维细胞的增殖起关键作用。
