Rechsteiner M, Rogers S W
Department of Biochemistry, University of Utah, Salt Lake City 84132, USA.
Trends Biochem Sci. 1996 Jul;21(7):267-71.
In 1986, we proposed that polypeptide sequences enriched in proline (P), glutamic acid (E), serine (S) and threonine (T) target proteins for rapid destruction. For much of the past decade there were only sporadic experimental tests of the hypothesis. This situation changed markedly during the past two years with a number of papers providing strong evidence that PEST regions do, in fact, serve as proteolytic signals. Here, we briefly review the properties of PEST regions and some interesting examples of the conditional nature of such signals. Most of the article, however, focuses on recent experimental support for the hypothesis and on mechanisms responsible for the rapid degradation of proteins that contain PEST regions.
1986年，我们提出富含脯氨酸（P）、谷氨酸（E）、丝氨酸（S）和苏氨酸（T）的多肽序列可作为靶蛋白快速降解的信号。在过去十年的大部分时间里，对这一假说仅有零星的实验验证。在过去两年中，这种情况发生了显著变化，多篇论文提供了有力证据，证明PEST区域实际上确实充当蛋白水解信号。在此，我们简要回顾PEST区域的特性以及此类信号具有条件性的一些有趣实例。然而，本文大部分内容聚焦于该假说最近获得的实验支持以及含PEST区域蛋白快速降解的相关机制。
