Rubin R H, Baltimore D, Chen B K, Wilkinson R A, Fischman A J
Center for Experimental Pharmacology and Therapeutics, Harvard University-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7460-3. doi: 10.1073/pnas.93.15.7460.
The tissue distribution of CD4 lymphocytes in normal C57/BL mice and CD4 knockout mice was determined by biodistribution measurements and gamma camera imaging with an 111In-labeled rat IgG2b monoclonal antibody directed against the murine CD-4 antigen. In normal mice high concentrations of antibody accumulated in the spleen and lymph nodes. At 45 hr after injection, the concentration of radiolabel in the spleen and lymph nodes of normal mice were 10- to 20-fold greater than in the corresponding tissue of the CD4 knockout mice and nonlymphoid tissues of both types of mice. At 24 and 45 hr, gamma camera images showed high concentrations of radiolabeled antibody in lymph node and spleen of normal but not knockout mice. These results indicate that radioimmunoscintigraphy with 111In-anti-CD4 is an excellent method for studying tissue distribution of CD lymphocytes in mice. Using an equivalent anti-human CD antibody, this method might be useful for studying the pathophysiology of conditions in which these cells play a critical role and for monitoring therapies for these disorders.
采用生物分布测量和γ相机成像技术，用一种针对鼠CD-4抗原的111In标记的大鼠IgG2b单克隆抗体，测定正常C57/BL小鼠和CD4基因敲除小鼠中CD4淋巴细胞的组织分布。在正常小鼠中，高浓度的抗体聚集在脾脏和淋巴结中。注射后45小时，正常小鼠脾脏和淋巴结中的放射性标记物浓度比CD4基因敲除小鼠相应组织以及两种小鼠的非淋巴组织中的浓度高10至20倍。在24小时和45小时时，γ相机图像显示正常小鼠而非基因敲除小鼠的淋巴结和脾脏中有高浓度的放射性标记抗体。这些结果表明，用111In-抗CD4进行放射免疫闪烁显像，是研究小鼠CD淋巴细胞组织分布的一种极佳方法。使用等效的抗人CD抗体，该方法可能有助于研究这些细胞起关键作用的疾病的病理生理学，并监测这些疾病的治疗情况。
