Naclerio G, Baccigalupi L, Zilhao R, De Felice M, Ricca E
Institute of Food Science and Technology, Consiglio Nazionale delle Ricerche, Avellino, Italy.
J Bacteriol. 1996 Aug;178(15):4375-80. doi: 10.1128/jb.178.15.4375-4380.1996.
Endospores of Bacillus subtilis are encased in a protein shell, known as the spore coat, composed of a lamella-like inner layer and an electron-dense outer layer. We report the identification and characterization of a gene, herein called cotH, located at 300 degrees on the B. subtilis genetic map between two divergent cot genes, cotB and cotG. The cotH open reading frame extended for 1,086 bp and corresponded to a polypeptide of 42.8 kDa. Spores of a cotH null mutant were normally heat, lysozyme, and chloroform resistant but were impaired in germination. The mutant spores were also pleiotropically deficient in several coat proteins, including the products of the previously cloned cotB, -C, and -G genes. On the basis of the analysis of a cotE cotH double mutant, we infer that CotH is probably localized in the inner coat and is involved in the assembly of several proteins in the outer layer of the coat.
枯草芽孢杆菌的芽孢被包裹在一层蛋白质外壳中，即芽孢衣，它由一层类片层状的内层和一层电子致密的外层组成。我们报告了一个基因的鉴定和特征，在此称为cotH，它位于枯草芽孢杆菌遗传图谱上300度处，在两个不同的cot基因cotB和cotG之间。cotH开放阅读框延伸1086 bp，对应一个42.8 kDa的多肽。cotH基因缺失突变体的芽孢通常对热、溶菌酶和氯仿具有抗性，但在萌发方面存在缺陷。突变体芽孢在几种芽孢衣蛋白方面也表现出多效性缺陷，包括先前克隆的cotB、-C和-G基因的产物。基于对cotE cotH双突变体的分析，我们推断CotH可能定位于内层芽孢衣，并参与外层芽孢衣中几种蛋白质的组装。
