Saggese Anella, Scamardella Veronica, Sirec Teja, Cangiano Giuseppina, Isticato Rachele, Pane Francesca, Amoresano Angela, Ricca Ezio, Baccigalupi Loredana
Department of Biology, Federico II University of Naples, Naples, Italy.
Department of Chemistry, Federico II University of Naples, Naples, Italy.
PLoS One. 2014 Aug 12;9(8):e104900. doi: 10.1371/journal.pone.0104900. eCollection 2014.
Spore formers are bacteria able to survive harsh environmental conditions by differentiating a specialized, highly resistant spore. In Bacillus subtilis, the model system for spore formers, the recently discovered crust and the proteinaceous coat are the external layers that surround the spore and contribute to its survival. The coat is formed by about seventy different proteins assembled and organized into three layers by the action of a subset of regulatory proteins, referred to as morphogenetic factors. CotH is a morphogenetic factor needed for the development of spores able to germinate efficiently and involved in the assembly of nine outer coat proteins, including CotG. Here we report that CotG has negative effects on spore germination and on the assembly of at least three outer coat proteins. Such negative action is exerted only in mutants lacking CotH, thus suggesting an antagonistic effect of the two proteins, with CotH counteracting the negative role of CotG.
产芽孢菌是一类细菌，它们能够通过分化出一种特殊的、高度抗性的芽孢来在恶劣环境条件下存活。在枯草芽孢杆菌（产芽孢菌的模式系统）中，最近发现的芽孢外壳和蛋白质外壳是围绕芽孢的外层结构，有助于芽孢的存活。外壳由大约70种不同的蛋白质组成，这些蛋白质在一组被称为形态发生因子的调控蛋白的作用下组装并组织成三层。CotH是一种形态发生因子，对于能够高效萌发的芽孢的发育是必需的，并且参与包括CotG在内的九种外壳蛋白的组装。在此我们报告，CotG对芽孢萌发以及至少三种外壳蛋白的组装具有负面影响。这种负面作用仅在缺乏CotH的突变体中发挥，因此表明这两种蛋白质具有拮抗作用，即CotH抵消了CotG的负面作用。
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