Warren G, McKown R, Marin A L, Teutonico R
DNA Plant Technology Corp., Oakland, California 94608, USA.
Plant Physiol. 1996 Aug;111(4):1011-9. doi: 10.1104/pp.111.4.1011.
We screened for mutations deleterious to the freezing tolerance of Arabidopsis thaliana (L.) Heynh. ecotype Columbia. Tolerance was assayed by the vigor and regrowth of intact plants after cold acclimation and freezing. From a chemically mutagenized population, we obtained 13 lines of mutants with highly penetrant phenotypes. In 5 of these, freezing sensitivity was attributable to chilling injury sustained during cold acclimation, but in the remaining 8 lines, the absence of injury prior to freezing suggested that they were affected specifically in the development of freezing tolerance. In backcrosses, freezing sensitivity from each line segregated as a single nuclear mutation. Complementation tests indicated that the 8 lines contained mutations in 7 different genes. The mutants' freezing sensitivity was also detectable in the leakage of electrolytes from frozen leaves. However, 1 mutant line that displayed a strong phenotype at the whole-plant level showed a relatively weak phenotype by the electrolyte leakage assay.
我们筛选了对拟南芥（Arabidopsis thaliana (L.) Heynh.）生态型哥伦比亚的耐冻性有害的突变。通过冷驯化和冷冻后完整植株的活力和再生来测定耐受性。从化学诱变群体中，我们获得了13个具有高渗透表型的突变体系。其中5个体系中，冻害敏感性归因于冷驯化期间遭受的冷害，但在其余8个体系中，冷冻前无损伤表明它们在耐冻性发育中受到特异性影响。在回交中，每个体系的冻害敏感性作为单个核突变进行分离。互补试验表明，这8个体系在7个不同基因中含有突变。突变体的冻害敏感性在冷冻叶片的电解质渗漏中也可检测到。然而，1个在全株水平表现出强表型的突变体系通过电解质渗漏试验显示出相对较弱的表型。
