Wusteman M C, Macdonald I
Metabolism. 1977 Aug;26(8):921-30. doi: 10.1016/0026-0495(77)90011-7.
Mature male rats were given, orally, 14C-fructose either alone or in conjunction with dihydroxyacetone, glyceraldehyde, or glycerol. Glycerol was also administered with 14C-glucose in a dose equimolar with fructose. After 2 hr the rats were killed and the liver triglyceride fraction was separated. Estimations of the degree of incorporation of 14C from the radioactive labeled carbohydrate into the glycerol and fatty acid moieties were carried out. Dihydroxyacetone and glyceraldehyde reduced the incorporation of 14C-fructose into hepatic triglyceride to approximately equal extents, suggesting that both trioses contribute equally to alpha-glycerophosphate formation. Glycerol, however, brought about a profound increase in the amount of fructose converted to triglyceride. This effect of glycerol was not apparent to any measurable extent on glucose incorporation into triglyceride and none of the intermediates affected the proportional distribution of radioactive counts between the fatty acid and glycerol moieties of the triglyceride after 14C-fructose administration.
给成年雄性大鼠经口给予14C-果糖，单独给予或与二羟基丙酮、甘油醛或甘油联合给予。甘油也与14C-葡萄糖以与果糖等摩尔的剂量一起给予。2小时后处死大鼠，分离肝脏甘油三酯部分。对放射性标记碳水化合物中的14C掺入甘油和脂肪酸部分的程度进行了测定。二羟基丙酮和甘油醛将14C-果糖掺入肝脏甘油三酯的程度降低到大致相同的程度，这表明两种丙糖对α-甘油磷酸的形成贡献相等。然而，甘油使转化为甘油三酯的果糖量显著增加。甘油的这种作用在任何可测量的程度上对葡萄糖掺入甘油三酯均不明显，并且在给予14C-果糖后，没有一种中间产物影响甘油三酯的脂肪酸和甘油部分之间放射性计数的比例分布。
