Gigliani F, Longo F, Gaddini L, Battaglia P A
Dipartimento di Biopatologia Umana, Università La Sapienza, Roma, Italy.
Mol Gen Genet. 1996 Jul 26;251(6):628-34. doi: 10.1007/BF02174111.
The Enhancer of split and achaete-scute gene complexes [E(spl)-C and AS-C] encode helix-loop-helix proteins required for neurogenesis in Drosophila. Using a heterologous bacterial system, we show that (i) the bHLH domains of the proteins encoded by the two gene complexes differ in their ability to form homo- and/or heterodimers; (ii) the bHLH domains of the E(spl)-C proteins m5, m7 and m8 interact with the bHLH domains of the Ac and Sc proteins. These bHLH domains form an interaction network which may represent the molecular mechanism whereby the competent state of the proneural cells is maintained until the terminal determination to neuroblast occurs. Also, the pattern of interactions of the bHLH domains of certain proteins encoded by the two gene complexes may explain their functional redundancy.
分裂增强子和无刚毛-毛缘基因复合体[E(spl)-C和AS-C]编码果蝇神经发生所需的螺旋-环-螺旋蛋白。利用异源细菌系统，我们发现：(i) 这两个基因复合体编码的蛋白质的bHLH结构域在形成同二聚体和/或异二聚体的能力上存在差异；(ii) E(spl)-C蛋白m5、m7和m8的bHLH结构域与Ac和Sc蛋白的bHLH结构域相互作用。这些bHLH结构域形成了一个相互作用网络，这可能代表了一种分子机制，通过该机制，神经前体细胞的感受态得以维持，直到最终确定成为神经母细胞。此外，这两个基因复合体编码的某些蛋白质的bHLH结构域的相互作用模式可能解释了它们的功能冗余。
