Lee L A, Alvarez K, Gross T, Harley J B
Department of Dermatology, University of Oklahoma Health Sciences Center, U.S.A.
J Invest Dermatol. 1996 Aug;107(2):225-8. doi: 10.1111/1523-1747.ep12329677.
The hY RNAs form a macromolecular complex with the 60-kDa Ro protein and may in addition be bound by the La protein. In this study, we examine the autoantibody responses to the intact protein-RNA complexes in 18 subacute cutaneous lupus (SCLE), 10 discoid lupus (DLE), and 18 neonatal lupus erythematosus (NLE) sera. All SCLE and NLE serum samples precipitated all four of the Ro hY RNAs, whereas none of the DLE serum samples precipitated the hY RNAs. Among the SCLE and NLE sera, there was a significant association between the amount of Ro hY RNAs precipitated and the concurrent presence of anti-La antibodies in the sera (p = 0.008), consistent with the hypothesis that both the 60-kDa Ro and the La proteins can bind the Ro hY RNAs. There was no correlation between the amount of hY RNAs precipitated and the titer of antibodies to the 60-kDa Ro protein in enzyme-linked immunosorbent assay (ELISA). This may be due to a difference in the epitopes formed by the antigen in the respective assays. The autoantibody response to Ro in SCLE and NLE, which is generally detectable by both immunodiffusion and ELISA, is directed to all the subsets of the Ro protein-hY RNA complexes. The autoantibody response in DLE, though frequently detectable by ELISA, is not of sufficient concentration or affinity to precipitate the Ro protein-RNA complexes. The autoantibody response to Ro in SCLE and NLE may include antibodies to epitopes created by the complexing of the 60-kDa Ro protein with hY RNA.
hY RNA与60 kDa的Ro蛋白形成大分子复合物，此外还可能与La蛋白结合。在本研究中，我们检测了18例亚急性皮肤型红斑狼疮（SCLE）、10例盘状红斑狼疮（DLE）和18例新生儿红斑狼疮（NLE）血清对完整蛋白质-RNA复合物的自身抗体反应。所有SCLE和NLE血清样本均沉淀出所有四种Ro hY RNA，而所有DLE血清样本均未沉淀出hY RNA。在SCLE和NLE血清中，沉淀的Ro hY RNA量与血清中同时存在的抗La抗体之间存在显著关联（p = 0.008），这与60 kDa的Ro蛋白和La蛋白均可结合Ro hY RNA的假设一致。在酶联免疫吸附测定（ELISA）中，沉淀的hY RNA量与60 kDa Ro蛋白抗体滴度之间无相关性。这可能是由于各自测定中抗原形成的表位存在差异。SCLE和NLE中对Ro的自身抗体反应通常可通过免疫扩散和ELISA检测到，该反应针对Ro蛋白-hY RNA复合物的所有亚群。DLE中的自身抗体反应虽然经常可通过ELISA检测到，但浓度或亲和力不足以沉淀Ro蛋白-RNA复合物。SCLE和NLE中对Ro的自身抗体反应可能包括针对60 kDa Ro蛋白与hY RNA复合形成的表位的抗体。
