Lee L A, Roberts C M, Frank M B, McCubbin V R, Reichlin M
Department of Dermatology, University of Oklahoma Health Sciences Center, Oklahoma City.
Arch Dermatol. 1994 Oct;130(10):1262-8.
Seventeen patients with subacute cutaneous lupus erythematosus (SCLE) were compared with 15 patients with discoid lupus erythematosus (DLE) to evaluate the relationship of 60- and 52-kd Ro/SSA autoantibodies to the clinical diagnosis and to evaluate assays for anti-Ro/SSA.
All serum samples from patients with SCLE had precipitating anti-Ro/SSA antibodies in immunodiffusion, and all had high titer anti-60-kd Ro/SSA in enzyme-linked immunosorbent assay. Immunoblotting was inadequately sensitive for detecting anti-60-kd Ro/SSA. Fifteen patients with SCLE had anti-52-kd Ro/SSA (11 high titer, four low titer). Only one of the 15 patients with DLE had precipitating, high-titer anti-Ro/SSA. Nine other patients with DLE had low-titer anti-60-kd Ro/SSA, and four had low-titer anti-52-kd Ro-SSA. Low-titer anti-Ro/SSA did not confer an increased risk for photosensitivity in the DLE group.
High-titer, precipitating antibodies to Ro/SSA are typical of SCLE and unusual in DLE. Low-titer, nonprecipitating antibodies to Ro/SSA are common in DLE and could be an indication of pathogenic factors shared with SCLE. However, low titers of anti-Ro/SSA do not confer a significant risk for SCLE skin lesions. For the purpose of clinical evaluation of skin disease, immunodiffusion assays for anti-Ro/SSA are cost-effective and informative.
将17例亚急性皮肤型红斑狼疮（SCLE）患者与15例盘状红斑狼疮（DLE）患者进行比较，以评估60kd和52kd Ro/SSA自身抗体与临床诊断的关系，并评估抗Ro/SSA检测方法。
SCLE患者的所有血清样本在免疫扩散试验中均有沉淀性抗Ro/SSA抗体，且在酶联免疫吸附测定中均有高滴度抗60kd Ro/SSA。免疫印迹法检测抗60kd Ro/SSA的敏感性不足。15例SCLE患者中有抗52kd Ro/SSA（11例高滴度，4例低滴度）。15例DLE患者中只有1例有沉淀性、高滴度抗Ro/SSA。其他9例DLE患者有低滴度抗60kd Ro/SSA，4例有低滴度抗52kd Ro-SSA。低滴度抗Ro/SSA在DLE组中并未增加光敏反应风险。
高滴度、沉淀性Ro/SSA抗体是SCLE的典型表现，在DLE中不常见。低滴度、非沉淀性Ro/SSA抗体在DLE中常见，可能表明与SCLE有共同的致病因素。然而，低滴度抗Ro/SSA不会显著增加SCLE皮肤病变的风险。为了对皮肤病进行临床评估，抗Ro/SSA的免疫扩散试验具有成本效益且信息丰富。
