Kristensen N M, Hoyne G F, Hayball J D, Hetzel C, Bourne T, Lamb J R
Infection and Immunity Section, Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK.
Int Immunol. 1996 Jul;8(7):1091-8. doi: 10.1093/intimm/8.7.1091.
In this study we demonstrate that immunization of H-2(b) mice with the allergen Der p 1 induces MHC class II restricted T cells that proliferate to residues 15-29 of Der p 1 (p15-29) and to the murine MHC class II-associated invariant chain derived peptide (CLIP). T cells from naive H-2(b) mice and those immunized with murine CLIP fail to respond to either CLIP or p15-29. T cell lines and clones reactive with p15-29 strongly proliferate in response to splenic antigen-presenting cells (APC) from normal H-2(b) mice but show reduced proliferation to APC from invariant chain deficient mice. Furthermore, T cells isolated from Der p 1 primed mice and expanded on H-2(b) spleen cells in the absence of the p15-29 epitope retained specificity for both p15-29 and CLIP, suggesting that naturally presented self components can act as mimetic peptides and may maintain T cell memory to foreign antigens.
在本研究中，我们证明用变应原Der p 1免疫H-2(b)小鼠可诱导出MHC II类限制性T细胞，这些T细胞可增殖至Der p 1的15 - 29位残基（p15 - 29）以及鼠MHC II类相关恒定链衍生肽（CLIP）。来自未免疫的H-2(b)小鼠的T细胞以及用鼠CLIP免疫的T细胞对CLIP或p15 - 29均无反应。与p15 - 29反应的T细胞系和克隆对来自正常H-2(b)小鼠的脾抗原呈递细胞（APC）有强烈增殖反应，但对来自恒定链缺陷小鼠的APC增殖反应减弱。此外，从Der p 1致敏小鼠分离并在无p15 - 29表位的情况下在H-2(b)脾细胞上扩增的T细胞对p15 - 29和CLIP均保持特异性，这表明天然呈递的自身成分可作为模拟肽，并可能维持对外源抗原的T细胞记忆。
