Erickson L D, Tygrett L T, Bhatia S K, Grabstein K H, Waldschmidt T J
Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA.
Int Immunol. 1996 Jul;8(7):1121-9. doi: 10.1093/intimm/8.7.1121.
Previous studies have established the distribution, biochemistry and functional attributes of human CD22, a B cell-restricted glycoprotein. Recently, molecular cloning of the murine CD22 equivalent revealed this molecule to be the same as the previously described Lyb8 alloantigen. Using the anti-Lyb8 mAb Cy34.1.2, the present report documents the expression patterns of CD22 within the murine B cell compartment. The results demonstrate that in the bone marrow, murine CD22 is absent on the surface of pro-B cells, pre-B cells and newly emerging IgM+ B cells. CD22 is present at a low density on immature IgMhi B cells and fully expressed on mature recirculating B cells. In the periphery, murine CD22 is expressed at mature levels on all B cell subsets including follicular, marginal zone, B1 and switched B cells. Further studies showed CD22 to be retained on activated murine B cells for extended periods. Finally, in combination with CD23 and heat stable antigen, CD22 can be used to delineate the immature splenic B cells, and distinguish them from follicular and marginal zone cells. Together, the results demonstrate murine CD22 to be a useful pan marker for all mature B cell subsets.
先前的研究已经确定了人类CD22（一种B细胞限制性糖蛋白）的分布、生物化学特性和功能属性。最近，对小鼠CD22等效物的分子克隆表明，该分子与先前描述的Lyb8同种异体抗原有相同之处。本报告使用抗Lyb8单克隆抗体Cy34.1.2记录了CD22在小鼠B细胞区室中的表达模式。结果表明，在骨髓中，前B细胞、前B细胞和新出现的IgM+B细胞表面不存在小鼠CD22。CD22在未成熟的IgMhi B细胞上以低密度存在，并在成熟的循环B细胞上完全表达。在外周，小鼠CD22在所有B细胞亚群（包括滤泡、边缘区、B1和转换B细胞）上以成熟水平表达。进一步的研究表明，CD22在活化的小鼠B细胞上长时间保留。最后，与CD23和热稳定抗原结合，CD22可用于描绘未成熟的脾脏B细胞，并将它们与滤泡和边缘区细胞区分开来。总之，结果表明小鼠CD22是所有成熟B细胞亚群的有用泛标记物。
