Muir P, Nicholson F, Spencer G T, Ajetunmobi J F, Starkey W G, Khan M, Archard L C, Cairns N J, Anderson V E, Leigh P N, Howard R S, Banatvala J E
Department of Virology, United Medical and Dental Schools of Guy's and St Thomas's Hospitals, London SE1 7EH, UK.
J Gen Virol. 1996 Jul;77 ( Pt 7):1469-76. doi: 10.1099/0022-1317-77-7-1469.
We have searched, using a sensitive nested-PCR, for enterovirus RNA in cerebrospinal fluid and post mortem central nervous system (CNS) tissue from patients with previous poliomyelitis with or without late functional deterioration, patients with motor neuron disease (MND), and control patients with other neurological disease or without neurological disease. Enterovirus RNA was detected in patients with previous poliomyelitis and MND, but also in control patients with and without neurological disease. Our results do not provide any evidence that such enterovirus infection is related to late functional deterioration in patients with previous poliomyelitis, which could be attributed to other medical conditions in most instances, and do not support the hypothesis that MND is associated with enterovirus infection of the CNS. Nucleotide sequence analysis of enterovirus RNA sequences detected indicated that enteroviruses detected were of the non-polio type.
我们采用灵敏的巢式聚合酶链反应，在患有既往脊髓灰质炎且有或无晚期功能恶化的患者、运动神经元病（MND）患者以及患有其他神经系统疾病或无神经系统疾病的对照患者的脑脊液和死后中枢神经系统（CNS）组织中搜索肠道病毒RNA。在患有既往脊髓灰质炎和MND的患者中检测到了肠道病毒RNA，但在有和没有神经系统疾病的对照患者中也检测到了。我们的结果没有提供任何证据表明这种肠道病毒感染与既往脊髓灰质炎患者的晚期功能恶化有关，在大多数情况下，晚期功能恶化可能归因于其他医疗状况，并且不支持MND与CNS肠道病毒感染相关的假说。对检测到的肠道病毒RNA序列进行的核苷酸序列分析表明，检测到的肠道病毒属于非脊髓灰质炎型。
