Sagawa H, Ohshima A, Kato I, Okuno Y, Isegawa Y
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd, 3-4-1 Seta, Otsu, Shiga 520-21, Japan.
J Gen Virol. 1996 Jul;77 ( Pt 7):1483-7. doi: 10.1099/0022-1317-77-7-1483.
A deletion mutant of influenza virus haemagglutinin (HA; headless HA) lacking the globular region was expressed in CV-1 cells and detected with a monoclonal antibody, C179, which recognizes a conformational epitope in the middle of the stem region of HA and neutralizes all H1 and H2 subtypes. The cDNA coding for the headless HA was constructed from influenza virus A/Okuda/57 (H2N2), which was also used to select C179. The conformational epitope recognized by C179 was highly stable even after removal of the globular region. The survival rate of mice immunized with the headless HA and challenged with lethal influenza virus A/FM/l/47 (H1N1) was significantly higher than that of the control mice. The headless HA has the potential to induce cross-protection against influenza virus infection.
一种缺乏球状区域的流感病毒血凝素缺失突变体（HA；无头HA）在CV-1细胞中表达，并用单克隆抗体C179进行检测，该抗体识别HA茎区中部的一个构象表位，并中和所有H1和H2亚型。编码无头HA的cDNA由甲型流感病毒A/Okuda/57（H2N2）构建而成，该病毒也用于筛选C179。即使去除球状区域后，C179识别的构象表位仍高度稳定。用无头HA免疫并用致死性甲型流感病毒A/FM/l/47（H1N1）攻击的小鼠存活率显著高于对照小鼠。无头HA有潜力诱导针对流感病毒感染的交叉保护作用。
