Carvalho C, Geslin P, Vaz Pato M V
Service de Résistance aux Antibiotiques, Bactériologie, Institut National de Santé, Lisbonne, Portugal.
Pathol Biol (Paris). 1996 May;44(5):430-4.
During a multicenter surveillance study on Streptococcus pneumoniae resistant to antibiotics in Portugal we have analyzed, within the predominant serotypes of penicillin G resistant strains, the chromosomal DNA restriction profiles by pulsed field gel electrophoresis. The aim of this work was to compare the PFGE restriction profiles of penicillin resistant pneumococci isolated in France with those of Portuguese strains within each serotype. We have studied fifteen penicillin resistant strains isolated in different geographical regions of France, and representing equally the three predominant resistant serotypes 23F, 9V and 14. In general we observed an important homogeneity of profiles within each serotype, in the two countries. In serotype 23F, the five French strains studied show subtypes of a pattern called A, also detected in the majority of 23F Portuguese strains; similarly 4 of the 5 serotype 9V strains belong to subtypes of the pattern G, also common within 9V Portuguese strains; 4 of the 5 strains of serotype 14 show a subtype of profile H, found often in Portuguese strains. The observed homogeneity of chromosomal DNA restriction profiles within each serotype in the two countries, confirms the importance of geographic spread of pneumococcal resistant clones, previously described.
在葡萄牙开展的一项关于肺炎链球菌抗生素耐药性的多中心监测研究中，我们在对青霉素G耐药菌株的主要血清型进行分析时，采用脉冲场凝胶电泳技术分析了染色体DNA限制酶切图谱。这项工作的目的是比较法国分离的青霉素耐药肺炎球菌与葡萄牙菌株在每种血清型中的PFGE限制酶切图谱。我们研究了在法国不同地理区域分离的15株青霉素耐药菌株，它们分别代表了三种主要的耐药血清型23F、9V和14。总体而言，我们观察到在这两个国家，每种血清型内的图谱具有重要的同质性。在血清型23F中，所研究的5株法国菌株显示出一种称为A的模式的亚型，在大多数23F葡萄牙菌株中也检测到了该亚型；同样，5株血清型9V菌株中的4株属于模式G的亚型，在9V葡萄牙菌株中也很常见；血清型14的5株菌株中有4株显示出图谱H的亚型，在葡萄牙菌株中经常发现。在这两个国家中，每种血清型内观察到的染色体DNA限制酶切图谱的同质性，证实了先前描述的肺炎球菌耐药克隆地理传播的重要性。
