子宫内膜癌DNA含量及细胞周期阶段分析的研究

[A study on DNA content and cell cycle phase analysis in endometrial carcinoma].

作者信息

Xue F, Jiao S, Zhao F

机构信息

General Hospital of Tianjin Medical University.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 1996 Apr;31(4):216-9.

PMID:8758776

Abstract

OBJECTIVE

To study the prognostic value of DNA content, cell cycle phase in endometrial carcinoma.

METHODS

DNA content and cell cycle phase analysis were performed on 100 paraffin-embedded archival specimens from 103 cases of endometrial carcinoma using flow cytometry.

RESULTS

Aneuploidy was found in 71.0% of specimens. The aneuploidy rate in specimens from grade III, deep myometrial invasion and stage III, IV was significantly higher than that in specimens from grade I, nonmyometrial invasion and stage I (P < 0.05 respectively). 5-year survival rate for patients with aneuploidy was 65.9% and for patients with diploidy was 87.6% (P < 0.01). High S-phase fraction (SPF) was associated with high grade, deep myometrial invasion and advanced stage. The mean SPF was 13.4% in the living group and 17% in the dead group. The 5-year survival rate for patients with SPF > 17% was 50.4% and for patients with SPF < 17% was 79.1% (P < 0.01). SPF and stage were the most important prognostic factors in endometrial carcinoma by Cox's proportional hazards model.

CONCLUSIONS

DNA ploidy and SPF were found to be of important prognostic indicators in endometrial carcinoma.

摘要

目的

研究DNA含量及细胞周期时相在子宫内膜癌中的预后价值。

方法

采用流式细胞术对103例子宫内膜癌患者的100份石蜡包埋存档标本进行DNA含量及细胞周期时相分析。

结果

71.0%的标本存在非整倍体。Ⅲ级、肌层浸润深度深及Ⅲ、Ⅳ期标本的非整倍体率显著高于Ⅰ级、无肌层浸润及Ⅰ期标本（P值分别<0.05）。非整倍体患者的5年生存率为65.9%，二倍体患者为87.6%（P<0.01）。高S期分数（SPF）与高级别、肌层浸润深度深及晚期相关。存活组的平均SPF为13.4%，死亡组为17%。SPF>17%患者的5年生存率为50.4%，SPF<17%患者为79.1%（P<0.01）。根据Cox比例风险模型，SPF和分期是子宫内膜癌最重要的预后因素。