Guan X Y, Xu J, Anzick S L, Zhang H, Trent J M, Meltzer P S
Laboratory of Cancer Genetics, National Center for Human Genome Research, NIH, Bethesda, Maryland 20892-4470, USA.
Cancer Res. 1996 Aug 1;56(15):3446-50.
In human breast carcinomas, increased copy number of DNA sequences derived from the long arm of chromosome 20 (20q) has been commonly observed by both chromosome microdissection and comparative genomic hybridization. This chromosomal region is likely to contain one or more genes that are the biological targets of this amplification event. We describe here the utilization of a chromosome microdissection-hybrid selection strategy to isolate transcribed sequences from microdissected homogeneously staining regions encompassing 20q. Using this strategy, we have isolated three novel amplified genes (termed AIB1, AIB3, and AIB4) from a cDNA library constructed from the 20q amplified breast cancer cell line BT-474. These three genes were mapped to 20q11 (AIB3 and AIB4) and 20q12 (AIB1) by fluorescence in situ hybridization. Our results indicate an unsuspected complexity to the amplification pattern of 20q in breast cancer and provide probes that will be useful for further characterization of tumor specimens.
在人类乳腺癌中，通过染色体显微切割和比较基因组杂交技术，经常观察到源自20号染色体长臂（20q）的DNA序列拷贝数增加。该染色体区域可能包含一个或多个基因，这些基因是这种扩增事件的生物学靶点。我们在此描述了一种染色体显微切割-杂交筛选策略的应用，以从包含20q的显微切割均匀染色区中分离转录序列。利用该策略，我们从由20q扩增的乳腺癌细胞系BT-474构建的cDNA文库中分离出三个新的扩增基因（称为AIB1、AIB3和AIB4）。通过荧光原位杂交将这三个基因定位到20q11（AIB3和AIB4）和20q12（AIB1）。我们的结果表明，乳腺癌中20q的扩增模式存在意想不到的复杂性，并提供了有助于进一步表征肿瘤标本的探针。
