苯并噻二唑通过激活系统获得性抗性信号转导途径诱导拟南芥产生抗病性。

Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway.

作者信息

Lawton K A, Friedrich L, Hunt M, Weymann K, Delaney T, Kessmann H, Staub T, Ryals J

机构信息

Ciba-Geigy Agricultural Biotechnology, Research Triangle Park, NC 27709-2257, USA.

出版信息

Plant J. 1996 Jul;10(1):71-82. doi: 10.1046/j.1365-313x.1996.10010071.x.

DOI:10.1046/j.1365-313x.1996.10010071.x

PMID:8758979

Abstract

Benzothiadiazole (BTH) is a novel chemical activator of disease resistance in tobacco, wheat and other important agricultural plants. In this report, it is shown that BTH works by activating SAR in Arabidopsis thaliana. BTH-treated plants were resistant to infection by turnip crinkle virus, Pseudomonas syringae pv 'tomato' DC3000 and Peronospora parasitica. Chemical treatment induced accumulation of mRNAs from the SAR-associated genes, PR-1, PR-2 and PR-5. BTH treatment induced both PR-1 mRNA accumulation and resistance against P. parasitica in the ethylene response mutants, etr1 and ein2, and in the methyl jasmonate-insensitive mutant, jar1, suggesting that BTH action is independent of these plant hormones. BTH treatment also induced both PR-1 mRNA accumulation and P. parasitica resistance in transgenic Arabidopsis plants expressing the nahG gene, suggesting that BTH action does not require salicylic acid accumulation. However, because BTH-treatment failed to induce either PR-1 mRNA accumulation or P. parasitica resistance in the non-inducible immunity mutant, nim1, it appears that BTH activates the SAR signal transduction pathway.

摘要

苯并噻二唑（BTH）是烟草、小麦及其他重要农作物中一种新型的抗病激活剂。本报告表明，BTH通过激活拟南芥中的系统获得性抗性（SAR）发挥作用。经BTH处理的植株对芜菁皱缩病毒、丁香假单胞菌番茄致病变种DC3000和寄生霜霉具有抗性。化学处理诱导了与SAR相关基因PR-1、PR-2和PR-5的mRNA积累。在乙烯反应突变体etr1和ein2以及茉莉酸甲酯不敏感突变体jar1中，BTH处理既诱导了PR-1 mRNA积累，又诱导了对寄生霜霉的抗性，这表明BTH的作用不依赖于这些植物激素。在表达nahG基因的转基因拟南芥植株中，BTH处理也诱导了PR-1 mRNA积累和对寄生霜霉的抗性，这表明BTH的作用不需要水杨酸积累。然而，由于BTH处理在非诱导性免疫突变体nim1中未能诱导PR-1 mRNA积累或对寄生霜霉的抗性，因此似乎BTH激活了SAR信号转导途径。