Kussovski V, Radoucheva T, Najdenski H, Cherepova N
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia Bulgaria.
Cytobios. 1995;84(338-339):157-69.
The photosensitive activity of haematoporphyrin (HP) and proflavine (PF) on some biological parameters of Salmonella dublin cells was assessed. The investigations showed a decreased respiratory activity of photosensitized PF bacterial cells, accompanied by lower virulence. HP-treatment and light irradiation of salmonellae did not influence their survival in vitro, which was in contrast to the PF-incubated and irradiated cells. Light irradiation of HP- and PF-treated bacteria did not change their phagocytosis from guinea pig alveolar macrophages. In the presence of visible light the PF-treatment considerably reduced the survival rate and multiplication in alveolar macrophages in comparison with HP-treated and light-exposed bacteria. Correlation was established between the degree of structural damage, as observed by electron microscopy and the level of diminution of the chosen biological parameters, which were more strongly expressed after PF-treatment. PF as a photosensitizer which influences the bacterial genomes and its possible practical use, is discussed.
评估了血卟啉（HP）和硫酸原黄素（PF）对都柏林沙门氏菌细胞某些生物学参数的光敏活性。研究表明，经光敏化的PF细菌细胞呼吸活性降低，毒力也较低。对沙门氏菌进行HP处理和光照，并不影响其体外存活率，这与经PF孵育和光照的细胞情况相反。对经HP和PF处理的细菌进行光照，并未改变豚鼠肺泡巨噬细胞对它们的吞噬作用。与经HP处理和光照的细菌相比，在可见光存在的情况下，PF处理显著降低了肺泡巨噬细胞中的存活率和增殖率。通过电子显微镜观察到的结构损伤程度与所选生物学参数的降低水平之间建立了相关性，PF处理后这些参数的表达更为强烈。文中讨论了PF作为影响细菌基因组的光敏剂及其可能的实际应用。
