一种用于确定基因组操作构建体中Cre或FLP重组靶点功能的简单检测方法。
A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.
作者信息
Buchholz F, Angrand P O, Stewart A F
机构信息
European Molecular Biology Laboratory, Gene Expression Program, Heidelberg, Germany.
出版信息
Nucleic Acids Res. 1996 Aug 1;24(15):3118-9. doi: 10.1093/nar/24.15.3118.
Abstract
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
摘要
我们报告了两种表达Cre重组酶或FLP重组酶的大肠杆菌菌株（294-Cre和294-FLP）的构建。含有任一重组酶（loxP位点或FRT位点）真实识别靶点的质粒在相应菌株中繁殖时会发生重组。因此，294-Cre和294-FLP为设计用于Cre或FLP介导的基因组操作的构建体的重组能力提供了一个简单的测试方法。
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本文引用的文献
1
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Cell. 1993 Jun 18;73(6):1155-64. doi: 10.1016/0092-8674(93)90644-6.
2
Site-specific recombinases: tools for genome engineering.位点特异性重组酶：基因组工程工具
Trends Genet. 1993 Dec;9(12):413-21. doi: 10.1016/0168-9525(93)90104-p.
3
Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting.利用细胞类型特异性基因靶向技术删除T细胞中的DNA聚合酶β基因片段。
Science. 1994 Jul 1;265(5168):103-6. doi: 10.1126/science.8016642.
4
A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination.通过Cre-loxP重组在胚胎干细胞中诱导产生的位点特异性染色体易位。
Nat Genet. 1995 Apr;9(4):376-85. doi: 10.1038/ng0495-376.
5
Inducible gene targeting in mice.小鼠中的诱导型基因靶向
Science. 1995 Sep 8;269(5229):1427-9. doi: 10.1126/science.7660125.
6
Ligand-regulated site-specific recombination.
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5940-4. doi: 10.1073/pnas.92.13.5940.
7
Genome engineering: the new mouse genetics.基因组工程：新的小鼠遗传学
Nat Med. 1995 Jun;1(6):592-4. doi: 10.1038/nm0695-592.
8
Chromosome engineering in mice.小鼠染色体工程
Nature. 1995 Dec 14;378(6558):720-4. doi: 10.1038/378720a0.
9
Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.2 微米环状位点特异性重组：最小底物及侧翼序列的可能作用。
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10
New method for generating deletions and gene replacements in Escherichia coli.在大肠杆菌中产生缺失和基因替换的新方法。
J Bacteriol. 1989 Sep;171(9):4617-22. doi: 10.1128/jb.171.9.4617-4622.1989.
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