Koskiniemi Hanna, Grocholski Thadee, Schneider Gunter, Niemi Jarmo
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):256-9. doi: 10.1107/S1744309109001389. Epub 2009 Feb 14.
12-deoxy-nogalonic acid oxygenase (SnoaB) catalyzes the oxygenation of 12-deoxy-nogalonic acid at position 12 to yield nogalonic acid, which is one of the steps in the biosynthesis of the polyketide nogalamycin in Streptomyces nogalater. SnoaB belongs to a family of small cofactor-free oxygenases which carry out oxygenation reactions without the aid of any prosthetic group, cofactor or metal ion. Recombinant SnoaB was crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 58.8, b = 114.1, c = 49.5 A, and these crystals diffracted to 2.4 A resolution. Recombinant SnoaB does not contain any methionine residues and three double mutants were designed and produced for the preparation of selenomethionine-substituted samples. The selenomethionine-substituted mutant F40M/L89M crystallized in the same space group as the native enzyme.
12-脱氧诺加洛宁酸加氧酶（SnoaB）催化12-脱氧诺加洛宁酸在第12位的氧化反应，生成诺加洛宁酸，这是诺加链霉菌中聚酮化合物诺加霉素生物合成过程中的一个步骤。SnoaB属于一类无辅因子的小型加氧酶家族，它们在不借助任何辅基、辅因子或金属离子的情况下进行氧化反应。重组SnoaB在空间群P2(1)2(1)2中结晶，晶胞参数a = 58.8，b = 114.1，c = 49.5 Å，这些晶体的衍射分辨率达到2.4 Å。重组SnoaB不含任何甲硫氨酸残基，为制备硒代甲硫氨酸取代样品设计并制备了三个双突变体。硒代甲硫氨酸取代的突变体F40M/L89M与天然酶在相同的空间群中结晶。
