Pruzzo C, Crippa A, Bertone S, Pane L, Carli A
Istituto di Microbiologia, Facoltà di Medicina, Università di Ancona, Italy.
Microbiology (Reading). 1996 Aug;142 ( Pt 8):2181-6. doi: 10.1099/13500872-142-8-2181.
Vibrio alginolyticus is the only culturable vibrio associated with the chitinaceous carapace of the copepod Tigriopus fulvus (Fisher 1860) living in Ligurian coastal rock pools (Tyrrhenian Sea). The characteristics of the interaction between chitin particles and V. alginolyticus were studied by analysing strains isolated both from the copepod surface and from rock-pool water. The highest degree of attachment to chitin was observed at 20 degrees C, in the presence of 3% NaCl. Bacterial treatment with N-acetylglucosamine and pronase E caused a reduction in attachment of 52-62% and 77-94%, respectively. Chitin pretreatment with either wheat germ agglutinin or membrane proteins (MPs) from V. alginolyticus caused a reduction in attachment, of 50-57% and 53-70%, respectively. No inhibition was observed when bacteria were pretreated with D-glucose, D-fucose or D-fructose, or when chitin was pretreated with concanavalin A and Escherichia coli DH5 alpha MPs. V. alginolyticus MPs able to bind chitin were isolated and analysed by SDS-PAGE. Four chitin-binding proteins were visualized in all tested strains (53, 35, 20 and 14 kDa); in vivo these peptides may efficiently mediate V. alginolyticus attachment to chitin-containing substrates.
溶藻弧菌是唯一可培养的弧菌，与生活在利古里亚沿海岩池（第勒尼安海）中的挠足类动物富氏虎斑猛水蚤（费舍尔，1860年）的几丁质外壳相关。通过分析从桡足类动物表面和岩池水分离出的菌株，研究了几丁质颗粒与溶藻弧菌之间相互作用的特征。在20℃、3%氯化钠存在的条件下，观察到对几丁质的附着程度最高。用N-乙酰葡糖胺和链霉蛋白酶E处理细菌，分别使附着减少52%-62%和77%-94%。用小麦胚芽凝集素或溶藻弧菌的膜蛋白（MPs）对几丁质进行预处理，分别使附着减少50%-57%和53%-70%。当用D-葡萄糖、D-岩藻糖或D-果糖预处理细菌，或用伴刀豆球蛋白A和大肠杆菌DH5α MPs预处理几丁质时，未观察到抑制作用。分离出能够结合几丁质的溶藻弧菌MPs，并通过SDS-PAGE进行分析。在所有测试菌株中均可见四种几丁质结合蛋白（53、35、20和14 kDa）；在体内，这些肽可能有效地介导溶藻弧菌附着于含几丁质的底物。
