Vezzulli L, Pezzati E, Repetto B, Stauder M, Giusto G, Pruzzo C
Department of Biology (DIBIO), University of Genoa, Genoa, Italy.
Lett Appl Microbiol. 2008 Jan;46(1):119-25. doi: 10.1111/j.1472-765X.2007.02269.x. Epub 2007 Oct 16.
To investigate the role of surface membrane proteins (MP) to promote attachment to chitin particles and copepods of different environmental and clinical vibrios.
The role of surface MP to promote attachment to chitin particles and the copepod Tigriopus fulvus was investigated in several environmental and clinical Vibrio strains by inhibition test methods. Attachment to both substrates was significantly inhibited by homologous MP treatment in all strains and percentages of inhibition were comparable with the ones observed with N-acetyl glucosamine (GlcNAc). Sarkosyl-insoluble MP extracted from tested strains were added to chitin particles either in the presence or in the absence of GlcNAc and the fraction bound to chitin in both conditions was visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Chitin-binding proteins (CBP) defined as Sarkosyl-insoluble MP that bound chitin in the absence of GlcNAc but did not in the presence of the sugar were isolated in all strains.
CBP are common in both environmental and clinical Vibrio strains and they have an important general role in mediating cell interactions with chitin-containing surfaces.
The role of CBP should be taken into account when investigating environmental persistence of aquatic vibrios.
研究表面膜蛋白（MP）在促进不同环境和临床弧菌附着于几丁质颗粒和桡足类动物方面的作用。
通过抑制试验方法，研究了几种环境和临床弧菌菌株中表面MP在促进附着于几丁质颗粒和桡足类动物细角滨对虾方面的作用。在所有菌株中，同源MP处理均显著抑制了对两种底物的附着，抑制百分比与用N-乙酰葡糖胺（GlcNAc）观察到的相当。将从受试菌株中提取的不溶于十二烷基肌氨酸钠的MP添加到几丁质颗粒中，无论有无GlcNAc，两种条件下与几丁质结合的部分均通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行可视化。在所有菌株中均分离出了几丁质结合蛋白（CBP），其定义为在无GlcNAc时能结合几丁质但在有该糖存在时不能结合的不溶于十二烷基肌氨酸钠的MP。
CBP在环境和临床弧菌菌株中均很常见，它们在介导细胞与含几丁质表面的相互作用中具有重要的普遍作用。
在研究水生弧菌的环境持久性时，应考虑CBP的作用。
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