Montenegro T C, Miranda E A, Gilman R
Laboratorio de Parasitología, Universidad Peruana Cayetano Heredia, Lima, Peru.
Ann Trop Med Parasitol. 1996 Apr;90(2):145-55. doi: 10.1080/00034983.1996.11813038.
Two antibodies, one monoclonal and one polyclonal, were produced and used to identify Taenia solium eggs, using the enzyme-linked immuno-electrotransfer blot technique (EITB). Different life-stages of Taenia solium and T. saginata, including eggs from gravid proglottids recovered, post-treatment, from patients infected with the tapeworms, and eggs of Diphyllobothrium pacificum and Hymenolepis nana from other patients were tested with these antibodies. The monoclonal antibody only recognized the eggs and immature proglottids of T. solium. The polyclonal antibody, however, not only reacted with the eggs, cysticerci and immature proglottids of T. solium but also with the eggs and immature proglottids of T. saginata. The sensitivity and specificity of the EITB were both 100% using the monoclonal antibody but only 78% and 60%, respectively, using the polyclonal antibody. Diagnostic bands for T. solium eggs corresponded to proteins of 22.5 kDa using the polyclonal antibody and 22.5-37 kDa using the monoclonal antibody. Species-specific fluorescence was obtained with an anti-T. solium monoclonal antibody which bound to egg-derived oncospheres of T. solium but not to those of T. saginata.
制备了一种单克隆抗体和一种多克隆抗体，并利用酶联免疫电转移印迹技术（EITB）来鉴定猪带绦虫卵。用这些抗体检测了猪带绦虫和牛带绦虫的不同生活阶段，包括从感染绦虫的患者经治疗后回收的妊娠节片中的虫卵，以及来自其他患者的太平洋裂头绦虫和微小膜壳绦虫的虫卵。单克隆抗体仅识别猪带绦虫的虫卵和未成熟节片。然而，多克隆抗体不仅与猪带绦虫的虫卵、囊尾蚴和未成熟节片发生反应，还与牛带绦虫的虫卵和未成熟节片发生反应。使用单克隆抗体时，EITB的敏感性和特异性均为100%，而使用多克隆抗体时，敏感性和特异性分别仅为78%和60%。使用多克隆抗体时，猪带绦虫卵的诊断条带对应于22.5 kDa的蛋白质，使用单克隆抗体时对应于22.5 - 37 kDa的蛋白质。用一种抗猪带绦虫单克隆抗体获得了种特异性荧光，该抗体与猪带绦虫虫卵来源的六钩蚴结合，但不与牛带绦虫的六钩蚴结合。
