[大鼠实验性肝癌发生过程中增殖细胞核抗原和原癌基因的表达]
[Expression of PCNA and protooncogenes during experimental hepatocarcinogenesis in rats].
作者信息
Ruan Y, Wu Z, Yang M
机构信息
Department of Ultrastructural Pathology, Tongji Medical University, Wuhan.
出版信息
Zhonghua Bing Li Xue Za Zhi. 1996 Feb;25(1):39-40.
The expression of PCNA and the protooncogenes of ras family (N-ras, H-ras and Ki-ras) in hepatocarcinoma cells and the proliferating liver cells in precancerous, hyperplastic nodules during experimental hepatocarcinogenesis, induced by diethylnitrosamine in rat liver was observed immunohistochemically and by in situ hybridization. The results indicated that there was a positive expression of PCNA in both the carcinomas cells and precancerous liver cells. The amount of PCNA-positive cells exhibited a negative correlation with the amount of infiltrating mast cells surrounding carcinoma cell nests and the hyperplastic, precancerous nodules. These results basically coincided with that of the separate observation of the expression of protooncogenes in the same study.
采用免疫组织化学和原位杂交技术，观察了二乙基亚硝胺诱导大鼠肝癌发生过程中，肝癌细胞以及癌前增生性结节中增殖的肝细胞内增殖细胞核抗原（PCNA）和ras家族原癌基因（N-ras、H-ras和Ki-ras）的表达情况。结果显示，癌细胞和癌前肝细胞中均有PCNA阳性表达。PCNA阳性细胞数量与癌细胞巢及增生性癌前结节周围浸润性肥大细胞数量呈负相关。这些结果与同一研究中原癌基因表达的单独观察结果基本一致。
Zotero 插件