Bassey E O, Life P F, Catty D, Gaston J S, Kumararatne D S
Department of Immunology, University of Birmingham, UK.
Tuber Lung Dis. 1996 Apr;77(2):146-53. doi: 10.1016/s0962-8479(96)90029-5.
To evaluate and compare the lymphoproliferative response of human peripheral blood mononuclear cells (PBMC) to fractionated soluble extracts of Mycobacterium tuberculosis H37Rv (MTSE) and M. bovis bacille Calmette-Guérin (BCG) (MBSE), and thereby determine responses that correlate to infection, and to contrast antibody and T-cell responses.
Membrane blots of SDS-PAGE fractionated M. tuberculosis H37Rv and M. bovis BCG were employed for antibody immunoblotting and T-cell proliferative responses using sera and PBMC from seven tuberculous and seven BCG vaccinated control subjects.
The profiles of responses contrasted rather interestingly, with antibody and T-cells responding more to higher and lower molecular weight fractions respectively. T-cells responding to antigens in the 59-88 kDa region discriminated between tuberculous and BCG vaccinated controls (P < 0.05) even though the differences were more toward the 70-75 kDa fractions within the region in question. Responses to smaller molecular weight fractions of both MTSE and MBSE were high in direct contrast to antibody responses. Additionally, responses to MBSE in these regions were generally higher than for MTSE in vaccinated controls. The reverse was the case with tuberculous subjects where responses to MTSE were generally higher, though not sufficiently significant in enough of the tuberculous subjects to be considered discriminatory.
T-cell proliferative responses to mycobacterial antigens in the 59-88 kDa region, and particularly antigens in the 70-75 kDa region, can be an indication of infection with M. tuberculosis, as well as the basis for discriminating between active disease and vaccination with BCG.
评估并比较人外周血单个核细胞（PBMC）对结核分枝杆菌H37Rv（MTSE）和卡介苗（BCG）（MBSE）分级可溶性提取物的淋巴细胞增殖反应，从而确定与感染相关的反应，并对比抗体和T细胞反应。
使用来自7名结核病患者和7名接种卡介苗的对照受试者的血清和PBMC，对经SDS-PAGE分级的结核分枝杆菌H37Rv和卡介苗进行膜印迹，用于抗体免疫印迹和T细胞增殖反应。
反应谱形成了颇为有趣的对比，抗体和T细胞分别对较高和较低分子量级分反应更强。对59 - 88 kDa区域抗原产生反应的T细胞能够区分结核病患者和接种卡介苗的对照者（P < 0.05），尽管在所讨论区域内差异更倾向于70 - 75 kDa级分。与抗体反应形成直接对比的是，对MTSE和MBSE较小分子量级分的反应都很高。此外，在这些区域接种卡介苗的对照者对MBSE的反应通常高于对MTSE的反应。结核病患者的情况则相反，他们对MTSE的反应通常更高，尽管在足够多的结核病患者中差异不显著，不足以作为鉴别依据。
对59 - 88 kDa区域内分枝杆菌抗原，特别是70 - 75 kDa区域内抗原的T细胞增殖反应，可作为感染结核分枝杆菌的指标，也是区分活动性疾病和卡介苗接种的依据。
