Hui A B, Lo K W, Leung S F, Choi P H, Fong Y, Lee J C, Huang D P
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong.
Cancer Res. 1996 Jul 15;56(14):3225-9.
Loss of heterozygosity (LOH) on the long arm of chromosome 11 had been reported in many types of solid tumors. In this study, we investigated the LOH patterns of chromosome 11 on 52 primary nasopharyngeal carcinomas using 10 microsatellite polymorphic markers. The results revealed that 28 of the 52 cases (53.8%) demonstrated LOH on at least one of the nine 11q microsatellite loci studied. The highest frequencies of LOH were found at the two loci D11S2000 (36.1%) and D11S934 (34.5 %), both located at 11q22-24. Two distinct regions of deletion were found at 11q, with the first one defined by INT-2 and D11S900 at 11q13.3-22, and the second region located between D11S2000 and D11S934 at 11q22-24. The two deletion regions overlap with the common areas of deletion reported in other tumor types. This suggests the presence of multiple putative tumor suppressor genes on chromosome 11q that may play a role in the development of nasopharyngeal carcinomas.
11号染色体长臂杂合性缺失（LOH）已在多种实体瘤中被报道。在本研究中，我们使用10个微卫星多态性标记物，对52例原发性鼻咽癌患者11号染色体的LOH模式进行了研究。结果显示，52例患者中有28例（53.8%）在研究的9个11q微卫星位点中的至少一个位点上出现了LOH。在两个位点D11S2000（36.1%）和D11S934（34.5%）发现了最高频率的LOH，这两个位点均位于11q22 - 24。在11q发现了两个不同的缺失区域，第一个区域由11q13.3 - 22处的INT - 2和D11S900界定，第二个区域位于11q22 - 24处的D11S2000和D11S934之间。这两个缺失区域与其他肿瘤类型中报道的常见缺失区域重叠。这表明11q染色体上存在多个可能的肿瘤抑制基因，它们可能在鼻咽癌的发生发展中发挥作用。
