Aaron R K, Ciombor D M
Department of Orthopaedics, Brown University, Providence, Rhode Island, USA.
J Orthop Res. 1996 Jul;14(4):582-9. doi: 10.1002/jor.1100140412.
Endochondral ossification can be modulated by a number of biochemical and biophysical stimuli. This study uses the experimental model of decalcified bone matrix-induced endochondral ossification to examine the effect of one biophysical stimulus, an electromagnetic field, on chondrogenesis, calcification, and osteogenesis. A temporal acceleration and quantitative increase in sulfate incorporation, glycosaminoglycan content, and calcification suggests that the stimulation of endochondral ossification is due to an increase in extracellular matrix synthesis. The locus of that stimulation is identified in the mesenchymal stage of endochondral bone development, and stimulation at this stage is essential for accelerated bone formation. The data suggest that enhanced differentiation of mesenchymal stem cells present at this stage is most likely responsible for the increase in extracellular matrix synthesis and bone maturation.
软骨内成骨可受多种生化和生物物理刺激的调节。本研究采用脱钙骨基质诱导软骨内成骨的实验模型，以研究一种生物物理刺激——电磁场对软骨形成、钙化和成骨的影响。硫酸盐掺入、糖胺聚糖含量和钙化的时间加速和定量增加表明，软骨内成骨的刺激是由于细胞外基质合成增加所致。该刺激的位点在软骨内骨发育的间充质阶段被确定，且在此阶段的刺激对于加速骨形成至关重要。数据表明，此阶段存在的间充质干细胞的增强分化很可能是细胞外基质合成增加和骨成熟的原因。
