Kasper M, Lackie P, Haase M, Schuh D, Müller M
Institute of Pathology, Technical University of Dresden, Germany.
Virchows Arch. 1996 Jul;428(4-5):207-15. doi: 10.1007/BF00196692.
Cathepsin D expression has been assessed by immunohistochemistry and immunoelectron microscopy in fetal, normal adult and injured lungs of human beings. In addition to the well known positivity of alveolar macrophages and the bronchial epithelial cells, normal type I and to a lesser extent type II pneumocytes showed a granular, cytoplasmic staining pattern. Using immunogold labelling of lowicryl embedded human lung, cathepsin D was present in lysosomes of epithelial cells. Double immunofluorescence labelling employing type I and type II specific antibodies or lectins confirmed the epithelial staining for cathepsin D. At the terminal sac period during lung development cathepsin D appears in the alveolar epithelium. In fibrotic specimens, enhanced immunoreactivity was found in epithelial and non-epithelial cells. Proliferative epithelial formations were strongly stained with cathepsin D antibodies, whereas detached, desquamated epithelial cells were weakly positive or negative. We suggest that cathepsin D plays a role in the remodelling process during fibrogenesis.
已通过免疫组织化学和免疫电子显微镜对人类胎儿、正常成人及损伤肺组织中的组织蛋白酶D表达进行了评估。除了众所周知的肺泡巨噬细胞和支气管上皮细胞呈阳性外，正常的I型肺细胞以及程度较轻的II型肺细胞呈现出颗粒状的细胞质染色模式。利用对低温包埋的人肺组织进行免疫金标记，发现组织蛋白酶D存在于上皮细胞的溶酶体中。采用I型和II型特异性抗体或凝集素进行双重免疫荧光标记，证实了组织蛋白酶D的上皮染色。在肺发育的终末囊期，组织蛋白酶D出现在肺泡上皮中。在纤维化标本中，上皮细胞和非上皮细胞的免疫反应性增强。增殖性上皮结构被组织蛋白酶D抗体强烈染色，而脱落、剥脱的上皮细胞弱阳性或阴性。我们认为组织蛋白酶D在纤维生成过程中的重塑过程中发挥作用。
