Kasper M, Huber O, Grossmann H, Rudolph B, Tränkner C, Müller M
Institute of Pathology, Medical Faculty, Technical University Dresden, Germany.
Histochem Cell Biol. 1995 Nov;104(5):383-90. doi: 10.1007/BF01458132.
Affinity purified rabbit anti-mouse E-cadherin antibodies, reacting with diverse rat epithelia, were used to characterize epithelial changes in a radiation-induced fibrosis model of rat lung by immunoblotting techniques, immunoperoxidase and immunofluorescence microscopy. Immunostaining of normal rat lung tissues revealed a predominant staining of type II pneumocytes. Immunoelectron microscopy confirmed the immunohistochemical data of normal lung tissue obtained at the light microscopic level. In severely injured rat lung, we found enhanced immunoreactivity for E-cadherin at the surface of type I alveolar epithelial cells. The results suggest that E-cadherin is an adhesion molecule that is modulated after pathological alteration of the alveolar epithelium and that the antiserum may be useful for the characterization of normal and diseased rat epithelia.
用亲和纯化的兔抗小鼠E-钙黏蛋白抗体（可与多种大鼠上皮细胞发生反应），通过免疫印迹技术、免疫过氧化物酶和免疫荧光显微镜技术，对大鼠肺辐射诱导纤维化模型中的上皮变化进行表征。正常大鼠肺组织的免疫染色显示II型肺泡上皮细胞染色占主导。免疫电子显微镜证实了在光镜水平获得的正常肺组织的免疫组化数据。在严重受损的大鼠肺中，我们发现I型肺泡上皮细胞表面的E-钙黏蛋白免疫反应性增强。结果表明，E-钙黏蛋白是一种在肺泡上皮发生病理改变后被调节的黏附分子，并且该抗血清可能有助于表征正常和患病大鼠的上皮细胞。
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