Tada Y, Nakase M, Adachi T, Nakamura R, Shimada H, Takahashi M, Fujimura T, Matsuda T
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan.
FEBS Lett. 1996 Aug 12;391(3):341-5. doi: 10.1016/0014-5793(96)00773-9.
An antisense gene strategy was applied to suppress the 14-16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed-specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen-specific monoclonal antibody and a sequence-specific antisense RNA probe demonstrated that the 14-16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild-type rice. The high levels of reduction observed were stably inherited in at least three generations.
采用反义基因策略抑制成熟水稻种子中14 - 16 kDa过敏原基因的表达。通过电穿孔法将在一些水稻种子特异性启动子控制下产生16 kDa过敏原反义RNA的基因构建体导入水稻。使用过敏原特异性单克隆抗体和序列特异性反义RNA探针，对转基因水稻植株种子进行免疫印迹和RNA印迹分析，结果表明，几个转基因株系种子中的14 - 16 kDa过敏原蛋白及其转录本含量远低于亲本野生型水稻种子。观察到的高水平降低至少在三代中稳定遗传。
