Göbel T W, Chen C H, Cooper M D
Basel Institute for Immunology, Switzerland.
Eur J Immunol. 1996 Aug;26(8):1743-7. doi: 10.1002/eji.1830260812.
A candidate avian CD6 homolog is identified by the S3 monoclonal antibody. The S3 antigen exists in a phosphorylated glycoprotein form of 130 kDa and a nonphosphorylated form of 110 kDa. Removal of phosphate groups and N-linked carbohydrates indicates a 78-kDa protein core. During thymocyte differentiation, the gamma delta T cells do not express S3, whereas mature CD4+ and CD8+ cells of alpha beta lineage acquire S3 antigen. All alpha beta T cells in the blood and spleen express the S3 antigen at relatively high levels. In contrast, only the CD8+ subpopulation of gamma delta T cells in the spleen expresses the antigen and neither alpha beta nor gamma delta T cells in the intestinal epithelium express the S3 antigen. The S3 antigen is also found on embryonic splenocytes with a phenotypic profile characteristic of avian natural killer cells. The biochemical characteristics and this cellular expression pattern imply that the S3 antigen is the chicken CD6 homolog.
一种候选禽源CD6同源物由S3单克隆抗体鉴定出来。S3抗原以130 kDa的磷酸化糖蛋白形式和110 kDa的非磷酸化形式存在。去除磷酸基团和N-连接的碳水化合物后显示出一个78-kDa的蛋白质核心。在胸腺细胞分化过程中，γδ T细胞不表达S3，而αβ谱系的成熟CD4+和CD8+细胞获得S3抗原。血液和脾脏中的所有αβ T细胞都相对高水平地表达S3抗原。相比之下，脾脏中γδ T细胞的CD8+亚群才表达该抗原，而肠上皮中的αβ和γδ T细胞均不表达S3抗原。S3抗原也在具有禽自然杀伤细胞表型特征的胚胎脾细胞上被发现。其生化特性和这种细胞表达模式表明S3抗原是鸡的CD6同源物。
