Franke W W, Stehr S, Stumpp S, Kuhn C, Heid H, Rackwitz H R, Schnölzer M, Baumann R, Holzhausen H J, Moll R
Division of Cell Biology, German Cancer Research Center, Herdelberg, Germany.
Differentiation. 1996 Jul;60(4):245-50. doi: 10.1046/j.1432-0436.1996.6040245.x.
We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin, and also from all other actin isoforms. We show, by immunofluorescence and immunoperoxidase microscopy of tissue sections, that cardiac/fetal alpha-actin can be localized not only in cardiomyocytes but also in skeletal muscles and their satellite cells during regeneration. These mAbs are potentially valuable in developmental biology, for the characterization of tissue and cultured myogenic cells, in pathology, and for serodiagnosis.
我们描述了三种针对合成十肽产生的鼠单克隆抗体（mAb），该合成十肽代表肌节α-肌动蛋白心脏/胎儿异构体的氨基末端序列。当用于免疫印迹或组织学免疫定位时，这些单克隆抗体可将心脏/胎儿α-肌动蛋白与骨骼肌α-肌动蛋白区分开来，也可与所有其他肌动蛋白异构体区分开来。通过组织切片的免疫荧光和免疫过氧化物酶显微镜检查，我们发现心脏/胎儿α-肌动蛋白不仅可以定位在心肌细胞中，还可以定位在再生过程中的骨骼肌及其卫星细胞中。这些单克隆抗体在发育生物学、组织和培养的成肌细胞表征、病理学以及血清诊断方面具有潜在价值。
