Gerritsen M E, Shen C P, Atkinson W J, Padgett R C, Gimbrone M A, Milstone D S
Institute for Bone and Joint Disease and Cancer, Bayer Corporation, West Haven, Connecticut 06516-4175, USA.
Lab Invest. 1996 Aug;75(2):175-84.
Studies of capillary morphogenesis and angiogenesis in vitro have suggested a role for E-selectin (CD62E) in the process of differentiation into tube-like structures. Recent studies by our group and others have demonstrated that mice lacking E-selectin because of germline inactivation of the E-selectin gene by gene targeting are viable and fertile, without apparent deficiencies in vascular development. Murine lung endothelial cells from wild-type and E-selectin-deficient animals were isolated using an activation-dependent sterile sorting method, and differentiation into tube-like structures on sparse fibronectin, Matrigel, and collagen gels was compared. Both types of murine lung endothelial cells spontaneously organized to form multicellular tubes and extensive anastomotic networks. There were no major differences in either the time course of development or the general appearance of the multicellular cords or tube-like structures formed by murine lung endothelial cells from wild-type or E-selectin-deficient mice, although different patterns were observed on different extracellular matrices. These studies, thus, demonstrate that E-selectin is not required for morphogenesis of 3-dimensional vascular structures in vitro.
体外毛细血管形态发生和血管生成的研究表明，E-选择素（CD62E）在分化为管状结构的过程中发挥作用。我们小组和其他研究小组最近的研究表明，由于通过基因靶向使E-选择素基因发生种系失活而缺乏E-选择素的小鼠能够存活且可育，血管发育无明显缺陷。使用激活依赖性无菌分选方法分离野生型和E-选择素缺陷型动物的小鼠肺内皮细胞，并比较其在稀疏纤连蛋白、基质胶和胶原凝胶上分化为管状结构的情况。两种类型的小鼠肺内皮细胞均自发组织形成多细胞管和广泛的吻合网络。野生型或E-选择素缺陷型小鼠的肺内皮细胞形成多细胞索或管状结构的发育时间进程或总体外观均无重大差异，尽管在不同的细胞外基质上观察到不同的模式。因此，这些研究表明，体外三维血管结构的形态发生不需要E-选择素。
