Elick T A, Bauser C A, Fraser M J
Department of Biological Sciences, University of Notre Dame, IN 46556, USA.
Genetica. 1996 Jul;98(1):33-41. doi: 10.1007/BF00120216.
The piggyBac Lepidopteran transposable element moves from the cellular genome into infecting baculovirus genomes during passage of the virus in cultured TN-368 cells. We have constructed genetically tagged piggyBac elements that permit analysis of excision when transiently introduced on plasmids into the piggyBac-deficient Spodoptera frugiperda IPLB-SF21AE cell line. Precise excision of the element from these plasmids occurs at a higher frequency in the presence of a helper plasmid that presumably supplies the piggyBac transposase. The results suggest that the piggyBac transposon encodes a protein that functions to facilitate not only insertion, but precise excision as well. This is the first demonstration of piggyBac mobility from plasmid sources in uninfected Lepidopteran cells.
在病毒于培养的TN - 368细胞中传代期间，杆状病毒属鳞翅目转座元件piggyBac从细胞基因组转移至感染的杆状病毒基因组中。我们构建了基因标记的piggyBac元件，当将其通过质粒瞬时导入piggyBac缺陷的草地贪夜蛾IPLB - SF21AE细胞系时，可对其切除情况进行分析。在可能提供piggyBac转座酶的辅助质粒存在的情况下，该元件从这些质粒上的精确切除频率更高。结果表明，piggyBac转座子编码一种蛋白质，其功能不仅有助于插入，还能促进精确切除。这是piggyBac在未感染的鳞翅目细胞中从质粒来源进行移动的首次证明。
