Atkinson P W, Warren W D, O'Brochta D A
Division of Entomology, Commonwealth Scientific and Industrial Research Organization, Canberra, Australia.
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9693-7. doi: 10.1073/pnas.90.20.9693.
The hobo transposable element from Drosophila melanogaster was found to be capable of excision, resulting in donor sites unlike those reported for any other transposable element currently known in animals. These empty sites most closely resemble those left by the transposable elements Ac and Tam3 in Zea mays and Antirrhinum majus, respectively. Like Ac and Tam3, the hobo element was found to function in a distantly related species, in this case the housefly Musca domestica. Hobo excision in M. domestica was found not to require the expression of hobo-encoded transposase but instead appears to be driven by an endogenous hobo transposase-like activity. DNA sequences displaying high homology to the hobo transposase gene were isolated from the genome of M. domestica and appear to be part of a mobile-element system related to hobo, Ac, and Tam3.
人们发现，果蝇中的霍博转座元件能够进行切除，产生的供体位点与目前已知的动物中任何其他转座元件所报告的位点不同。这些空位分别与玉米和金鱼草中由转座元件Ac和Tam3留下的空位最为相似。与Ac和Tam3一样，霍博元件在远缘物种家蝇中也具有功能。在家蝇中，霍博元件的切除不需要霍博编码的转座酶的表达，而是似乎由一种内源性的类霍博转座酶活性驱动。从家蝇基因组中分离出了与霍博转座酶基因具有高度同源性的DNA序列，这些序列似乎是与霍博、Ac和Tam3相关的移动元件系统的一部分。
