O'Brochta D A, Warren W D, Saville K J, Atkinson P W
University of Maryland Biotechnology Institute, Center for Agricultural Biotechnology, College Park 20742.
Mol Gen Genet. 1994 Jul 8;244(1):9-14. doi: 10.1007/BF00280181.
A modified hobo element from Drosophila melanogaster was introduced into embryos of the housefly, Musca domestica (family Muscidae) and the Queensland fruitfly, Bactrocera tryoni (family Tephritidae) to assess its ability to transpose. Hobo was capable of transposition in these species and transposition products had all of the hallmarks of hobo transposition products recovered from D. melanogaster, including the movement only of sequences precisely delimited by the inverted terminal repeats of hobo, the creation of an 8 bp duplication of the insertion site and an absolute requirement for hobo-encoded transposase. Transposition of hobo into the target gene resulted in a non-random distribution of insertion sites, with 10 of 38 independent insertions into the same nucleotide position. The results indicate that hobo can transpose in heterologous species, further demonstrating the similarity of hobo to Ac (Activator) of Zea mays and Tam3 of Antirrhinum majus. Hobo has excellent potential to act as a gene vector or gene tagging agent in nondrosophilid insects.
将来自黑腹果蝇的一种修饰后的hobo元件导入家蝇（蝇科）和昆士兰实蝇（实蝇科）的胚胎中，以评估其转座能力。hobo能够在这些物种中转座，转座产物具有从黑腹果蝇中回收的hobo转座产物的所有特征，包括只有由hobo的反向末端重复序列精确界定的序列发生移动、在插入位点产生8 bp的重复以及对hobo编码的转座酶有绝对需求。hobo转座到靶基因中导致插入位点的非随机分布，38个独立插入中有10个插入到相同的核苷酸位置。结果表明，hobo可以在异源物种中转座，进一步证明了hobo与玉米的Ac（激活子）和金鱼草的Tam3的相似性。hobo在非果蝇昆虫中作为基因载体或基因标记剂具有极好的潜力。
