Voitenko V, Poulsen L K, Nielsen L, Norgaard A, Bindslev-Jensen C, Skov P S
Allergy Unit, IIR, RHIMA, National University Hospital, Copenhagen, Denmark.
Allergy. 1997 Feb;52(2):136-43. doi: 10.1111/j.1398-9995.1997.tb00967.x.
Our investigation aimed to produce and characterize a kiwi extract and to use this extract to investigate a possible cross-reactivity with birch pollen. Kiwi was extracted in two buffers: phosphate-buffered saline (PBS) and borate-buffered saline (BBS). Extraction in BBS produced a double amount of protein, and a more stabile extract. Tandem crossed-immunoelectrophoresis showed that the BBS and PBS extracts had several common, but also a few individual, proteins. The mixture of both extracts was assumed to represent the most complete allergen extract. The allergenic properties of the kiwi extract were investigated by immunoblotting (IB), RAST, and histamine-release (HR) test in 15 birch-pollen-allergic patients (eight of them with clinical kiwi allergy) and one with clinical monoallergy to kiwi. All eight birch-pollen-allergic patients with kiwi allergy and the kiwi-monoallergic patient were positive in kiwi IB binding most frequently to proteins of 10-12 and 20-25 kDa. With our extract, RAST was positive in four kiwi-allergic and one non-kiwi-allergic patient, whereas the HR test was positive in five kiwi-allergic patients and negative in all non-kiwi-allergic patients. RAST and IB inhibition demonstrated cross-reactivity between birch-pollen and kiwi allergens due to a 10-12 kDa protein. In conclusion, a kiwi extract with allergenic properties was produced, and, by the methods used, cross-reactivity was demonstrated between birch-pollen and kiwi allergens.
我们的研究旨在制备并表征猕猴桃提取物，并利用该提取物研究其与桦树花粉可能存在的交叉反应性。猕猴桃在两种缓冲液中进行提取：磷酸盐缓冲盐水（PBS）和硼酸盐缓冲盐水（BBS）。在BBS中提取得到的蛋白量是原来的两倍，且提取物更稳定。串联交叉免疫电泳显示，BBS提取物和PBS提取物有几种共同的蛋白质，但也有一些各自特有的蛋白质。两种提取物的混合物被认为代表了最完整的过敏原提取物。通过免疫印迹法（IB）、放射性过敏原吸附试验（RAST）和组胺释放试验（HR），对15名桦树花粉过敏患者（其中8人有临床猕猴桃过敏）和1名临床对猕猴桃单过敏的患者进行了猕猴桃提取物过敏特性的研究。所有8名对桦树花粉过敏且对猕猴桃过敏的患者以及对猕猴桃单过敏的患者，在猕猴桃免疫印迹试验中均呈阳性，最常与10 - 12 kDa和20 - 25 kDa的蛋白质结合。使用我们的提取物，放射性过敏原吸附试验在4名对猕猴桃过敏的患者和1名不对猕猴桃过敏的患者中呈阳性，而组胺释放试验在5名对猕猴桃过敏的患者中呈阳性，在所有不对猕猴桃过敏的患者中呈阴性。放射性过敏原吸附试验和免疫印迹抑制试验证明，由于一种10 - 12 kDa的蛋白质，桦树花粉和猕猴桃过敏原之间存在交叉反应性。总之，制备出了具有过敏特性的猕猴桃提取物，并且通过所采用的方法证明了桦树花粉和猕猴桃过敏原之间存在交叉反应性。
