Osterman L A, Sverdlova P S, Chupeeva V V
Ontogenez. 1977;8(3):228-37.
The chromatographic profiles of isoacceptor tRNA's for 9 amino acids from the loach embryos at two early developmental stages were compared by the method of chromatography on sepharose column in the decreasing gradient of ammonium sulphate concentration to elucidate the possible role of tRNA in the control of early embryogenesis processes. Certain differences both in the proportions of some isoacceptor peaks and their relative positions on the chromatographic profile were found for 8 aminoacyl tRNA. Methionyl-tRNA was represented by one constant peak.
采用硫酸铵浓度递减梯度的琼脂糖柱层析法,比较了泥鳅胚胎两个早期发育阶段9种氨基酸同功受体tRNA的色谱图谱,以阐明tRNA在早期胚胎发生过程控制中的可能作用。发现8种氨酰tRNA在某些同功受体峰的比例及其在色谱图谱上的相对位置上均存在差异。甲硫氨酰-tRNA由一个恒定峰代表。