Astafieva I, Maksimova I, Lukanidin E, Alakhov V, Kabanov A
Moscow Institute of Biotechnology, Inc. Faculty of Chemistry, Moscow State University, Russia.
FEBS Lett. 1996 Jul 8;389(3):278-80. doi: 10.1016/0014-5793(96)00607-2.
Polyelectrolyte complexes formed between DNA and poly(N-ethyl4-vinylpyridinium) cations were shown to effectively transfect mammalian cells [7]. This work suggests that the polycation-mediated uptake of the plasmid DNA and cell transfection are significantly enhanced when these complexes are administered simultaneously with a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) copolymer, Pluronic P85. The uptake studies were performed using radioactively labeled pRSV CAT plasmid on NIH 3T3, MDCK, and Jurkat cell lines. The transfection was investigated by chloramphenicol acetyltransferase assay using 3T3 cells as a model. The effects reported may be useful for the enhancement of the polycation-mediated cell transfection.
已表明，DNA与聚（N-乙基-4-乙烯基吡啶鎓）阳离子形成的聚电解质复合物能有效转染哺乳动物细胞[7]。这项研究表明，当这些复合物与聚（环氧乙烷）-嵌段-聚（环氧丙烷）-嵌段-聚（环氧乙烷）共聚物普朗尼克P85同时给药时，聚阳离子介导的质粒DNA摄取和细胞转染会显著增强。摄取研究使用放射性标记的pRSV CAT质粒在NIH 3T3、MDCK和Jurkat细胞系上进行。以3T3细胞为模型，通过氯霉素乙酰转移酶测定法研究转染情况。所报道的这些效应可能有助于增强聚阳离子介导的细胞转染。
