Brookes S J, Hennig G, Schemann M
Department of Human Physiology, Flinders University, Adelaide, South Australia.
J Comp Neurol. 1998 Jul 27;397(2):268-80. doi: 10.1002/(sici)1096-9861(19980727)397:2<268::aid-cne8>3.0.co;2-z.
The projections of enteric neurons to the circular muscle of the guinea pig gastric corpus were investigated systematically by using the retrogradely transported fluorescent carbocyanine dye 1,1'-didodecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate (DiI), applied to the muscle layer or myenteric plexus in vitro. DiI-labeled motor neuron cell bodies were located up to 6.3 mm aboral, 17 mm oral, and up to 20 mm circumferential to the DiI application site. Labeled nerve fibers ran for long distances from the DiI application site toward the greater and lesser curvatures, where they coursed parallel to the bundles of the "gastric sling" muscle. The majority of labeled cells were located toward the lesser curvature of the stomach. Nerve cell bodies that were aboral to the DiI application site were usually small, immunoreactive for choline acetyltransferase, and, thus, were likely to be excitatory motor neurons. Neurons that were located orally were larger, fewer in number, and immunoreactive for nitric oxide synthase and, thus, were likely to be inhibitory motor neurons. Application of DiI directly to the myenteric plexus filled neurons up to 15 mm aborally and up to 21 mm orally but labeled few neurons circumferentially. All nerve cells that were filled from either the circular muscle or the myenteric plexus had Dogiel type I morphological features. These results demonstrate a clear polarity of projection of inhibitory and excitatory motor neurons and a functionally continuous innervation of the circular and gastric sling muscle layers. Nonmotor neurons in the myenteric plexus were demonstrated, but neurons with Dogiel type II morphological features are apparently absent.
利用逆行运输的荧光花青染料1,1'-二癸基-3,3,3',3'-四甲基吲哚花青高氯酸盐（DiI），在体外将其应用于豚鼠胃体环行肌层或肌间神经丛，系统地研究了肠神经元向豚鼠胃体环行肌的投射。DiI标记的运动神经元胞体位于距DiI注射部位口侧17 mm、肛侧6.3 mm以及圆周方向20 mm范围内。标记的神经纤维从DiI注射部位向大弯和小弯方向长距离延伸，在那里它们与“胃悬带”肌束平行走行。大多数标记细胞位于胃小弯方向。位于DiI注射部位肛侧的神经细胞体通常较小，对胆碱乙酰转移酶呈免疫反应，因此可能是兴奋性运动神经元。位于口侧的神经元较大，数量较少，对一氧化氮合酶呈免疫反应，因此可能是抑制性运动神经元。将DiI直接应用于肌间神经丛可使肛侧15 mm和口侧21 mm范围内的神经元被填充，但圆周方向标记的神经元很少。从环行肌或肌间神经丛填充的所有神经细胞均具有I型多极神经元形态特征。这些结果表明抑制性和兴奋性运动神经元的投射具有明显的极性，并且环行肌层和胃悬带肌层在功能上具有连续的神经支配。肌间神经丛中的非运动神经元得到了证实，但显然不存在具有II型多极神经元形态特征的神经元。
