Price K J, Hanson P J, Whittle B J
Pharmaceutical Sciences Institute, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
Cell Tissue Res. 1996 Jul;285(1):157-63. doi: 10.1007/s004410050631.
Nitric oxide has been implicated in the regulation of blood flow, mucosal integrity and mucus secretion in the gastric mucosa. An antiserum directed against the C-terminal hexadecapeptide of rat brain nitric oxide synthase (NOS) and monoclonal antibodies to the neuronal and endothelial forms of NOS were used to establish the location of isoforms of NOS in rat gastric glandular mucosa. Antibodies to the neuronal form of NOS reacted with a band of 160 kDa on immunoblots of brain and gastric mucosa, and the addition of the hexadecapeptide inhibited recognition by the antipeptide antiserum. The antibody to endothelial NOS detected a band of 140 kDa on protein blots of samples of intestinal mesentery and gastric mucosa. Immunohistochemistry using these antibodies demonstrated that material related to neuronal NOS was present in surface cells of the gastric mucosa, and showed a similar localization to intense NADPH diaphorase activity. The antibody to endothelial NOS did not stain the surface of the gastric mucosa but recognized blood vessels in the lower region of the gastric glands and in the sub-mucosa. This study suggests that nitric oxide might act both as an intra- and inter-cellular messenger to regulate mucus release, and that the NOS present in surface cells is related more closely to the neuronal than to the endothelial isoform.
一氧化氮与胃黏膜的血流调节、黏膜完整性及黏液分泌有关。使用一种针对大鼠脑一氧化氮合酶（NOS）C末端十六肽的抗血清以及针对神经元型和内皮型NOS的单克隆抗体来确定NOS同工型在大鼠胃腺黏膜中的定位。针对神经元型NOS的抗体在脑和胃黏膜的免疫印迹上与一条160 kDa的条带发生反应，加入十六肽可抑制抗肽抗血清的识别。针对内皮型NOS的抗体在肠系膜和胃黏膜样本的蛋白质印迹上检测到一条140 kDa的条带。使用这些抗体进行免疫组织化学显示，与神经元型NOS相关的物质存在于胃黏膜的表面细胞中，并且其定位与强烈的NADPH黄递酶活性相似。针对内皮型NOS的抗体未对胃黏膜表面进行染色，但识别胃腺下部区域和黏膜下层的血管。这项研究表明，一氧化氮可能作为细胞内和细胞间信使来调节黏液释放，并且表面细胞中存在的NOS与神经元型而非内皮型同工型关系更为密切。
