Shah S W, McGadey J, Moore M R, Houston T, Payne A P
Laboratory of Human Anatomy, University of Glasgow, UK.
J Comp Physiol B. 1996;166(2):144-9. doi: 10.1007/BF00301178.
Porphyrin biosynthesis was examined in the Harderian gland of the female golden hamster by fluorometric assays of gland porphyrin content and by measuring the activity of a rate-limiting enzyme for haem biosynthesis, delta-aminolaevulinic acid synthase. Both porphyrin content and enzyme activity are high in normal female glands. Enzyme activity was lowered in females ovariectomised for 6 weeks, and both enzyme activity and porphyrin content were greatly lowered in ovariectomised females given the dopamine agonist bromocriptine; this suppression could be prevented by simultaneous prolactin administration. Bromocriptine (but not ovariectomy alone) also masculinised the morphology of the Harderian gland, resulting in the appearance of type II cells and polytubular complexes; again, the simultaneous administration of prolactin prevented masculinisation. The results support the hypothesis that while androgens have an inhibitory effect on porphyrin synthesis within this model, prolactin may have a major facilitatory role.
通过对雌性金黄仓鼠哈德氏腺卟啉含量进行荧光测定，并测量血红素生物合成的限速酶δ-氨基-γ-酮戊酸合酶的活性，对哈德氏腺中的卟啉生物合成进行了研究。正常雌性腺体中的卟啉含量和酶活性均较高。切除卵巢6周的雌性动物酶活性降低，而给予多巴胺激动剂溴隐亭的去卵巢雌性动物的酶活性和卟啉含量均大幅降低；同时给予催乳素可防止这种抑制作用。溴隐亭（但单独卵巢切除不会）还使哈德氏腺的形态雄性化，导致II型细胞和多管复合体出现；同样，同时给予催乳素可防止雄性化。结果支持了这样的假说：在该模型中，雄激素对卟啉合成有抑制作用，而催乳素可能起主要的促进作用。
