Marr F A, Shah S W, McGadey J, Moore M R, Houston T, Payne A P
Department of Anatomy, University of Glasgow, UK.
J Comp Physiol B. 1995;164(7):524-9. doi: 10.1007/BF00261392.
Porphyrin biosynthesis was examined in the Harderian gland of the male golden hamster by fluorometric assays of gland porphyrin content and by measuring the activity of a rate-limiting enzyme for haem biosynthesis, 5-aminolaevulinic acid synthase. Both porphyrin content and enzyme activity are low in normal male glands but were greatly raised in males castrated for 6 weeks. However, porphyrin synthesis remained at basal levels in castrates given the dopamine agonist bromocriptine; this suppression could be reversed by simultaneous prolactin administration, and castrated males receiving prolactin alone exhibited very high enzyme activity and porphyrin content. Bromocriptine also prevents the morphological feminisation of the Harderian gland which would normally occur after castration; again, the simultaneous administration of prolactin permits feminisation to occur. The results support the hypothesis that, while androgens have an inhibitory effect on porphyrin synthesis within this model, other factors, including prolactin, are permissive.
通过荧光法测定雄性金黄仓鼠哈德氏腺中的卟啉含量，并测量血红素生物合成的限速酶5-氨基乙酰丙酸合成酶的活性，来研究哈德氏腺中的卟啉生物合成。正常雄性腺体中的卟啉含量和酶活性都很低，但在阉割6周的雄性中，两者都大幅升高。然而，给予多巴胺激动剂溴隐亭的去势动物的卟啉合成仍维持在基础水平；同时给予催乳素可逆转这种抑制作用，单独接受催乳素的去势雄性表现出非常高的酶活性和卟啉含量。溴隐亭还可防止哈德氏腺在阉割后通常会出现的形态女性化；同样，同时给予催乳素会使女性化发生。结果支持这样的假说：在这个模型中，虽然雄激素对卟啉合成有抑制作用，但包括催乳素在内的其他因素则起允许作用。
